Agronomy for Sustainable Development

, Volume 29, Issue 4, pp 497–501

Detection of transgenic cp4 epsps genes in the soil food web

  • Miranda M. Hart
  • Jeff R. Powell
  • Robert H. Gulden
  • David J. Levy-Booth
  • Kari E. Dunfield
  • K. Peter Pauls
  • Clarence J. Swanton
  • John N. Klironomos
  • Jack T. Trevors
Research Article

DOI: 10.1051/agro/2009020

Cite this article as:
Hart, M.M., Powell, J.R., Gulden, R.H. et al. Agron. Sustain. Dev. (2009) 29: 497. doi:10.1051/agro/2009020

Abstract

The persistence and movement of transgenic DNA in agricultural and natural systems is largely unknown. This movement poses a threat of horizontal gene transfer and possible proliferation of genetically modified DNA into the general environment. To assess the persistence of transgenic DNA in a field of Roundup Ready® corn, we quantified the presence of the transgene for glyphosate tolerance within a soil food web. Using quantitative real-time PCR, we identified the cp4 epsps transgene in bulk soil microarthropods, nematodes, macroarthropods and earthworms sampled within the corn cropping system. We found evidence of the transgene at all dates and in all animal groups. Transgenic DNA concentration in animal was significantly higher than that of background soil, suggesting the animals were feeding directly on transgenic plant material. It remains to be tested whether this DNA was still within the plant residues, present as free, extracellular DNA or had already undergone genetic transformation into competent bacterial cells. These results are the first to demonstrate the persistence of transgenic crop DNA residues within a food web.

agriculture DNA environment corn foodweb glyphosate soil transgenic 

Copyright information

© Springer S+B Media B.V. 2009

Authors and Affiliations

  • Miranda M. Hart
    • 1
  • Jeff R. Powell
    • 1
  • Robert H. Gulden
    • 2
  • David J. Levy-Booth
    • 3
  • Kari E. Dunfield
    • 4
  • K. Peter Pauls
    • 2
  • Clarence J. Swanton
    • 2
  • John N. Klironomos
    • 1
  • Jack T. Trevors
    • 3
  1. 1.Department of Integrative BiologyUniversity of GuelphGuelphCanada
  2. 2.Department of Plant AgricultureUniversity of GuelphGuelphCanada
  3. 3.Department of Environmental BiologyUniversity of GuelphGuelphCanada
  4. 4.Department of Land Resource ScienceUniversity of GuelphGuelphCanada