Ecological Research

, Volume 15, Issue 3, pp 263–269

Microsatellite analysis of pollen flow in Rhododendron metternichii var. hondoense

  • Yoshiaki Kameyama
  • Yuji Isagi
  • Kazuaki Naito
  • Nobukazu Nakagoshi
Original Articles

DOI: 10.1046/j.1440-1703.2000.00347.x

Cite this article as:
Kameyama, Y., Isagi, Y., Naito, K. et al. Ecol Res (2000) 15: 263. doi:10.1046/j.1440-1703.2000.00347.x

Microsatellite analysis was used to characterize the patterns of pollen flow in a 150 m × 70 m quadrat containing 18 flowering trees of Rhododendron metternichii Sieb. et Zucc. var. hondoense Nakai. Using six microsatellite genetic markers and exclusion analysis, we determined the paternal trees of 216 seedlings germinated from five fruits produced by four adults. Each fruit was pollinated by a small number of largely adjacent trees, but 20–30% of pollen came from outside the quadrat. Adult trees that produced many flowers had a high self-pollination rate. A directional flow of pollen from late-blooming trees to early ones was observed.

Key words

microsatellite genetic marker paternity analysis pollen-mediated gene flow self pollination subpopulations 

Copyright information

© Blackwell Science Asia Pty. Ltd. 2000

Authors and Affiliations

  • Yoshiaki Kameyama
    • 1
  • Yuji Isagi
    • 2
  • Kazuaki Naito
    • 3
  • Nobukazu Nakagoshi
    • 1
  1. 1.Graduate School for International Development and CooperationHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Laboratory of Silviculture, Kansai Research CenterForestry and Forest Products Research InstituteKyotoJapan
  3. 3.Rural Ecology and Wildlife Conservation Division, Institute of Natural and Environmental SciencesHimeji Institute of TechnologyToyookaJapan
  4. 4.Graduate School for International Development and CooperationHiroshima UniversityHigashi–HiroshimaJapan

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