Journal of Industrial Microbiology and Biotechnology

, Volume 22, Issue 1, pp 44-47

Shear stress effects on plant cell suspension cultures in a rotating wall vessel bioreactor

  • X SunAffiliated withDepartment of Chemical and Bioresource Engineering, Colorado State University, Fort Collins, CO 80523, USA
  • , J C LindenAffiliated withDepartment of Chemical and Bioresource Engineering, Colorado State University, Fort Collins, CO 80523, USA

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A rotating wall vessel, designed for growth of mammalian cells under microgravity, was used to study shear effects on Taxus cuspidata plant suspension cell cultures. Shear stress, as quantified by defined shear fields of Couette viscometers, improved specific cell growth rates and was detrimental to volumetric product formation rates.

Keywords: RWV; shear; Taxol®; Taxus; secondary metabolite; plant cell culture