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A series of laboratory exercises utilizing luxR gene of Vibrio fischeri and gfp gene of Aequoria victoria to teach the broad applications of polymerase chain reaction

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Journal of Industrial Microbiology and Biotechnology

Three experiments are described; directional cloning of the luxR gene from the bioluminescent marine bacterium, Vibrio fischeri, directional cloning of the gfpgene from the marine jelly fish, Aequoria victoria, and the construction of a LuxR-GFP fusion protein. Experiments are presented using lux and gfp in an undergraduate biology curriculum. Journal of Industrial Microbiology & Biotechnology (2000) 24, 345–352.

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Authors and Affiliations

  1. King’s College, Department of Biology, Wilkes-Barre, PA 18711, USA, , , , , , US

    J A Slock, P M Farrugia, T A Horn, J A Phillips, J T Smedley & M J Romanko

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  1. J A Slock
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  2. P M Farrugia
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  3. T A Horn
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  4. J A Phillips
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  5. J T Smedley
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  6. M J Romanko
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Received 02 April 1999/ Accepted in revised form 19 November 1999

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Slock, J., Farrugia, P., Horn, T. et al. A series of laboratory exercises utilizing luxR gene of Vibrio fischeri and gfp gene of Aequoria victoria to teach the broad applications of polymerase chain reaction. J Ind Microbiol Biotech 24, 345–352 (2000). https://doi.org/10.1038/sj.jim.2900803

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  • DOI: https://doi.org/10.1038/sj.jim.2900803

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