Journal of Industrial Microbiology and Biotechnology

, Volume 20, Issue 1, pp 61–68

Effect of the siderophore alcaligin E on the bioavailability of Cd to Alcaligenes eutrophus CH34

  • A Gilis
  • P Corbisier
  • W Baeyens
  • S Taghavi
  • M Mergeay
  • D van der Lelie

DOI: 10.1038/sj.jim.2900478

Cite this article as:
Gilis, A., Corbisier, P., Baeyens, W. et al. J Ind Microbiol Biotech (1998) 20: 61. doi:10.1038/sj.jim.2900478

Alcaligin E, the siderophore of the heavy metal-resistant A. eutrophus strain CH34, was shown to interact with Cd and consequently affect its bioavailability and toxicity. The addition of alcaligin E markedly stimulated the growth in the presence of Cd of an alcaligin E-deficient CH34 derivative. Using bioluminescence assays, this effect could be assigned to a decrease in bioavailability of Cd in the presence of alcaligin E. However, Cd-uptake studies showed no influence of alcaligin E on the cellular concentration of Cd. Furthermore, by scanning electron microscopy, the morphology of precipitated Cd crystals was shown to be altered by alcaligin E. These data suggest that alcaligin E, besides its function in iron supply to the cell, provides a protection against heavy metal toxicity. A link between the A. eutrophus CH34 siderophore system and the czc-mediated Cd-efflux system is hypothesized.

Keywords: siderophore; Alcaligenes eutrophus; heavy metal resistance; czc; bioavailability; biosensor

Copyright information

© Society for Industrial Microbiology 1998

Authors and Affiliations

  • A Gilis
    • 1
  • P Corbisier
    • 1
  • W Baeyens
    • 1
  • S Taghavi
    • 1
  • M Mergeay
    • 1
  • D van der Lelie
    • 1
  1. 1.Milieutechnologie, Vlaamse Instelling voor Technologisch Onderzoek (VITO), Boeretang 200, B-2400 Mol, BelgiumBE