Journal of Industrial Microbiology and Biotechnology

, Volume 19, Issue 2, pp 98–103

Effects of growth conditions on production of methyl selenides in cultures of Rhodobacter sphaeroides

  • V Van Fleet-Stalder
  • H Gürleyük
  • R Bachofen
  • T G Chasteen

DOI: 10.1038/sj.jim.2900423

Cite this article as:
Van Fleet-Stalder, V., Gürleyük, H., Bachofen, R. et al. J Ind Microbiol Biotech (1997) 19: 98. doi:10.1038/sj.jim.2900423

Rhodobacter sphaeroides

2.4.1 exposed to selenate or selenite produced volatile selenium compounds. Total amounts of dimethyl selenide, dimethyl diselenide, dimethyl sulfide and dimethyl disulfide in culture medium and headspace were determined. The highest selenate volatilization occurred in the late stationary phase of growth. However, cultures deprived of light in the stationary phase of growth produced much less of the volatile organo-selenium compounds. Lower culture pHs increased the rate of selenium volatilization. Low sulfate concentration limited biomass production and selenium volatilization; high sulfate concentrations had an enhancing effect on the release of organo-selenium compounds. Cultures of R. sphaeroides reacted very differently to amendments with increasing amounts of selenate and selenite. Only small amounts of selenite were volatilized; meanwhile high amounts of methylated selenides were found in selenate-poisoned cultures.

Keywords: Rhodobacter sphaeroides; selenate; selenite; reduction; methylation

Copyright information

© Society for Industrial Microbiology 1997

Authors and Affiliations

  • V Van Fleet-Stalder
    • 1
  • H Gürleyük
    • 1
  • R Bachofen
    • 2
  • T G Chasteen
    • 1
  1. 1.Department of Chemistry, Sam Houston State University, Huntsville, TX 77341, USAUS
  2. 2.Institute of Plant Biology/Microbiology, University of Zürich, CH-8008 Zürich, SwitzerlandCH