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Not Just a Plasma Membrane Protein: in Cardiac Muscle Cells Alpha-II Spectrin also Shows a Close Association with Myofibrils

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An Erratum to this article was published on 01 April 2005

Abstract

Spectrin and its associated proteins are essential for the integrity of muscle cells and there is increasing evidence for their involvement in signalling pathways as well as having a structural function in mediating stress. Spectrin is a multigene family and it is essential to determine which isoforms are present and their location in the cell. In heart muscle, we have found that one spectrin isoform, αII-spectrin, is strongly represented and, using immunofluorescence, we show that it lies within the contractile fibres near the Z-disc as well as on the cardiomyocyte plasma membrane. Electron microscopy of immunogold-labelled cryosections reveals statistically significant clustering of gold particles near the Z-disc, within and close to the edge of myofibrils. βII-spectrin and ankyrin-R and G are both known to occupy this region. We suggest that αIIβII spectrin tetramers with ankyrin organise and/or stabilise cardiac muscle cell membrane components relative to the contractile apparatus.

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Authors and Affiliations

  1. The Randall Centre for Molecular Mechanisms of Cell Function, New Hunt's House, King's College London, Guy's Campus, London, SE1 1UL, UK; Tel.:

    Pauline M. Bennett, Anthony J. Baines, Alison M. Maggs & Jennifer C. Pinder

  2. Department of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK

    Anthony J. Baines

  3. INSERM U409, Faculté de Médecine Xavier Bichat, BP 416, 16 rue Henri Huchard, F-75870, Paris Cedex 18, France

    Marie-Christine Lecomte

Authors
  1. Pauline M. Bennett
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  2. Anthony J. Baines
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  3. Marie-Christine Lecomte
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  4. Alison M. Maggs
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  5. Jennifer C. Pinder
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s10974-005-1496-y.

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Bennett, P.M., Baines, A.J., Lecomte, MC. et al. Not Just a Plasma Membrane Protein: in Cardiac Muscle Cells Alpha-II Spectrin also Shows a Close Association with Myofibrils. J Muscle Res Cell Motil 25, 119–126 (2004). https://doi.org/10.1023/B:JURE.0000035892.77399.51

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  • DOI: https://doi.org/10.1023/B:JURE.0000035892.77399.51

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