Article

Biotechnology Letters

, Volume 26, Issue 13, pp 1077-1088

Phospholipase-mediated preparation of 1-ricinoleoyl-2-acyl-sn- glycero-3-phosphocholine from soya and egg phosphatidylcholine

  • T. VijeetaAffiliated withDivision of Lipid Science and Technology, Indian Institute of Chemical Technology
  • , J.R.C. ReddyAffiliated withDivision of Lipid Science and Technology, Indian Institute of Chemical Technology
  • , B.V.S.K. RaoAffiliated withDivision of Lipid Science and Technology, Indian Institute of Chemical Technology
  • , M.S.L. KarunaAffiliated withDivision of Lipid Science and Technology, Indian Institute of Chemical Technology
  • , R.B.N. PrasadAffiliated withDivision of Lipid Science and Technology, Indian Institute of Chemical Technology

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Abstract

1-Ricinoleoyl-2-acyl-sn-glycero-3-phosphocholine was prepared by incorporating ricinoleic acid completely in the sn-1 position of egg and soya phosphatidylcholine (PC) using immobilized phospholipase A1 as the catalyst. The optimum reaction conditions for maximum incorporation of ricinoleic acid into PC through transesterification were 10% (w/w) immobilized enzyme (116 mg), a 1:5 mol ratio of PC (soya, 387 mg; egg, 384 mg) to methyl ricinoleate (780 mg) at 50 °C for 24 h in hexane.

egg lecithin phosphatidylcholine phospholipase A1 ricinoleic acid soya lecithin