Biologia Plantarum

, Volume 43, Issue 3, pp 381-386

First online:

Induction of Oxidative Stress and Antioxidant Activity by Hydrogen Peroxide Treatment in Tolerant and Susceptible Wheat Genotypes

  • R.K. SairamAffiliated withDivision of Plant Physiology, Indian Agricultural Research Institute Email author 
  • , G.C. SrivastavaAffiliated withDivision of Plant Physiology, Indian Agricultural Research Institute

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We induced an oxidative stress by means of exogenous hydrogen peroxide in two wheat genotypes, C 306 (tolerant to water stress) and Hira (susceptible to water stress), and investigated oxidative injury and changes in antioxidant enzymes activity. H2O2 treatment caused chlorophyll degradation, lipid peroxidation, decreased membrane stability and activity of nitrate reductase. Hydrogen peroxide increased the activity of antioxidant enzymes, glutathione reductase and catalase. These effects increased with increasing H2O2 concentrations. However, no change was observed in the activity of superoxide dismutase and proline accumulation.

active oxygen species catalase chloroplast glutathione reductase lipid peroxidation nitrate reductase proline superoxide dismutase Triticum aestivum