Skip to main content
SpringerLink
Log in

Nitric Oxide-Mediated Regulation of Gastric H+, K+-ATPase and Alcohol Dehydrogenase Following Ethanol-Induced Injury in Rats

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

The mucosal protective effect of nitric oxide(NO) was examined by usingNG-nitro-L-arginine methyl ester (L-NAME) asnitric oxide synthase (NOS) inhibitor and nitroprusside(NP) as NO donating agent, in ethanol-induced rat gastric lesion model.The results are summarized as follows: (1) As gastrictissue samples were examined by light microscopy,intragastric exposure of ethanol was demonstrated to induce gastric injury, which was more prominentin female rats. The depletion of NO by L-NAME treatmentexacerbated the ethanol-induced gastric lesion but NPtogether with ethanol promoted repair of the mucosal injury, especially in female rats. (2)Gastric H+, K+-ATPase enzymeactivity, which was responsible for acid secretion,seemed not to be effected by ethanol treatment. Togetherwith ethanol, L-NAME treatment activated, whereas NP treatmentinhibited, the enzyme activity in female rats. (3)Ethanol treatment inhibited gastric alcoholdehydrogenase (ADH) activity, which was responsible forthe first-pass metabolism of ethanol. Together with ethanol,L-NAME did not effect the enzyme activity whereas NPtreatment disappeared the inhibitory effect of ethanolin both gender. Hydroxyl radical (OH•) scavenger activity was found to increase in ethanol andethanol + NP groups in both sexes, but superoxideradical (O2 -•) scavengeractivity did not change. The results indicate that NOmay ameliorate the damaging effect of ethanol possibly by regulating acidsecretion, ethanol metabolism, and antioxidant contentin rat gastric mucosa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Oates PJ, Hakkinen JP: Studies on the mechanism of ethanolinduced gastric damage in rats. Gastroenterology 94:10–21, 1988

    Google Scholar 

  2. Klaassen CHW, Swarts HGP, De Pont JJHHM: Ethanol stimulate s expression of functional H+, K+-ATPase in SF9 cells.Biochem Biophys Res Commun 210:907–913, 1995

    Google Scholar 

  3. Bell AE, Sellers LA, Allen A, Cunliffe WJ, Morris ER, Ross-Murphy SB: Properties of gastric and duodenal mucus: effect of proteolysis, disulfide reduction, bile acid, ethanol, and hypertonicity on mucus gel structure. Gastroenterology 88:269–280, 1985

    Google Scholar 

  4. Nordman R, Ribiere C, Roach H: Implication of free radical mechanisms in ethanol-induced cellular injury. Free Radic Biol Med 12:219–240, 1992

    Google Scholar 

  5. Gislason H, Herfjord JK, Guttu K, Grong K, Gronbech JE, Ulvik R, Svanes K: Adaptive protection related to clearance of ethanol in gastric mucosa of cats. Scand J Gastroenterol 28:361–368, 1993

    Google Scholar 

  6. Konturek SJ, Brzozowski T, Stachura J, Dembinski A, Majka J: Role of gastric blood flow, neutrophil infiltration and mucosal cell proliferation in gastric adaptation to aspirin in rats. Gut 35:1189–1196, 1994

    Google Scholar 

  7. Takeuchi K, Kato S, Yashuhiro T, Yagi K: Mechanism of acid secretory change s in rat stomach after damage by taurocholate. Dig Dis Sci 42:645–653, 1997

    Google Scholar 

  8. Takeuchi K, Okabe S: Mechanism of gastric alkaline response in the stomach after damage. Dig Dis Sci 40(4):865–871, 1995

    Google Scholar 

  9. Takeuchi K, Takehara K, Kaneko T, Okabe S: Nitric oxide and prostaglandins in regulation of acid secretory response in rat stomach following injury. J Pharmacol Exp Ther 272:357–363, 1995

    Google Scholar 

  10. Brown JF, Keates AC, Hanson PJ, Whittle BJR: Nitric oxide generators and cGMP stimulate mucus secretion by rat gastric mucosal cells. Am J Physiol 265(Gastrointest Liver Physiol28):G418-G422, 1993

    Google Scholar 

  11. Kanwar S, Wallace JL, Befus D, Kubes P: Nitric oxide synthesis inhibition increases epithelial permeability via mast cells. Am J Physiol 266:G222-G229, 1994

    Google Scholar 

  12. Schmidt KL, Henagan JM, Mitchell PA, Smith GS, Miller TA: The protective effects of a prostaglandin without antisecretory properties against ethanol-induced injury in the rat stomach: A histologic study. Histol Histopathol 2:173–183, 1987

    Google Scholar 

  13. Nakao T, Tashima Y, Nagao K, et al: Highly specific sodiumpotassium-associated adenosine triphosphate from various tissues of rabbit. Biochem Biophys Res Commun. 19:755–758, 1965

    Google Scholar 

  14. Taussky HH, Shorr E: A micro colorimetric method for determination of inorganic phosphorus. J Biol Chem 202:675–685, 1953

    Google Scholar 

  15. Lowry OH, Rosebrough NJ, Farr AL, Randell RJ: Protein me asurement with Folin phenol reagent. J Biol Chem 193:265–275, 1951

    Google Scholar 

  16. Beauchamp C, Fridovich I: Superoxide dismutase: Improved assays and applicable to acrylamide gels. Anal Biochem 44:276–287, 1997

    Google Scholar 

  17. Arouma OI: Deoxyribose assay for detecting hydroxyl radicals. Methods Enzymol 233:57–66, 1994

    Google Scholar 

  18. Loguercio C, Taranto DE, Beneduce F, del Vecchio Blanco C, De Vincentiis A, Nardi G, Romano M: Glutathione prevents ethanol induced gastric mucosal damage and depletion of suflhydryl compounds in humans. Gut 34:161, 1993

    Google Scholar 

  19. Klaassen CHW, De Pont JJHHM: Gastric H+, K+-ATPase. Cell Physiol Biochem 4:115–134, 1994

    Google Scholar 

  20. Weiner CP, Lizasoain I, Baylis SA, Knowles RG, Charles IG, Moncada S: Induction of calcium-dependent nitric oxide synthases by sex hormones. Proc Nattl Acad Sci USA 91(11):5212–5216, 1994

    Google Scholar 

  21. Hayashi T, Yamada K, Esaki T, Kuzuya M, Satake S, Ishikawa T, Hidaka H, Iguchi A: Estrogen increases endothelial nitric oxide by a receptor-mediated system. Biochem Biophys Res Commun 214( 3):847–855, 1995

    Google Scholar 

  22. Julkunen RJK, Dipadove C, Lieber CS: First pass metabolism of ethanol— a gastrointestinal barrie r against the systemic toxicity of ethanol. Life Sci 37:567–573, 1985

    Google Scholar 

  23. Lee L, Schmidt KL, Tornwall MS, Henagan JM, Miller TA: Gender differences in ethanol oxidation and injury in the rat stomach. Alcohol 9:421–425, 1992

    Google Scholar 

  24. Ligumsky M, Sestieri M, Okon E, Ginsburg I: Antioxidants inhibit ethanol-induced gastric injury in the rat. Role of manganese, glycine, and carotene. Scand J Gastroenterol 30:854–860, 1995

    Google Scholar 

  25. Matsumoto T, Moriguchi R, Yamada H: Role of polymorphonuclear leucocytes and oxygen-derived free radicals in the formation of gastric lesions induced by HCl/ethanol, and a possible me chanism of protection by anti-ulcer polysaccharide. J Pharmacol 45:535–539, 1993

    Google Scholar 

  26. Goos SP, Hogg N, Kalyanaraman B: The antioxidant effect of spermine NONOate in human low density lipoprotein. Chem Res Toxicol 3:800–806, 1995

    Google Scholar 

  27. Grisham MB, von Ritten C, Smith BF, Lamont JT, Granger DN: Interaction between oxygen radicals and gastric mucin. Am J Physiol 253(Gastrointest Live r Physiol 16):G93-G96, 1987

    Google Scholar 

Download references

Authors
  1. R. Bulut
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Unlucerci
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Bekpinar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Kuntsal
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bulut, R., Unlucerci, Y., Bekpinar, S. et al. Nitric Oxide-Mediated Regulation of Gastric H+, K+-ATPase and Alcohol Dehydrogenase Following Ethanol-Induced Injury in Rats. Dig Dis Sci 44, 1417–1422 (1999). https://doi.org/10.1023/A:1026608020133

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026608020133

Search

Navigation