Pharmaceutical Research

, Volume 17, Issue 9, pp 1092–1097

Evaluation of a Human Bio-Engineered Skin Equivalent for Drug Permeation Studies

Authors

  • Charles Asbill
    • Department of Basic Pharmaceutical Sciences, College of PharmacyUniversity of South Carolina
  • Nanhye Kim
    • Department of Basic Pharmaceutical Sciences, College of PharmacyUniversity of South Carolina
  • Ayman El-Kattan
    • Department of Basic Pharmaceutical Sciences, College of PharmacyUniversity of South Carolina
  • Kim Creek
    • Children's Cancer Research Laboratory, Department of Pediatrics and Department of PathologyUniversity of South Carolina School of Medicine
  • Philip Wertz
    • Dows InstituteUniversity of Iowa
  • Bozena Michniak
    • Department of Basic Pharmaceutical Sciences College of PharmacyUniversity of South Carolina
Article

DOI: 10.1023/A:1026405712870

Cite this article as:
Asbill, C., Kim, N., El-Kattan, A. et al. Pharm Res (2000) 17: 1092. doi:10.1023/A:1026405712870

Abstract

Purpose. To test the barrier function of a bio-engineered human skin (BHS) using three model drugs (caffeine, hydrocortisone, and tamoxifen) in vitro. To investigate the lipid composition and microscopic structure of the BHS.

Methods. The human skin substitute was composed of both epidermal and dermal layers, the latter having a bovine collagen matrix. The permeability of the BHS to three model drugs was compared to that obtained in other percutaneous testing models (human cadaver skin, hairless mouse skin, and EpiDerm™). Lipid analysis of the BHS was performed by high performance thin layered chromatrography. Histological evalulation of the BHS was performed using routine H&E staining.

Results. The BHS mimicked human skin in terms of lipid composition, gross ultrastructure, and the formation of a stratum corneum. However, the permeability of the BHS to caffeine, hydrocortisone, and tamoxifen was 3-4 fold higher than that of human cadaver skin.

Conclusions. In summary, the results indicate that the BHS may be an acceptable in vitro model for drug permeability testing.

drug delivery systemsskin alternativestransdermal drug deliverypermeability

Copyright information

© Plenum Publishing Corporation 2000