Abstract
Fourteen yeast strains were screened for production of 2-phenylethanol from l-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l−1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-phenylethanol l−1 at 35 °C. The most productive strains were Kluyveromyces marxianus CBS 600 and CBS 397.
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References
Albertazzi E, Cardillo R, Servi S, Zucchi G (1994) Biogeneration of 2-phenylethanol and 2-phenylacetate important aroma components. Biotechnol. Lett. 16: 491–496.
Anonymous (1999) Natürliche Aromastoffe immer stärker gefragt. Z. Lebensmittelw. 50: 30–31.
Äyräpää T (1965) The formation of phenethyl alcohol from 14Clabelled phenylalanine. J. Inst. Brew. 71: 341–347.
Clark GS (1990) Phenethyl alcohol. Perfum. Flavor. 15: 37–44.
Ehrlich F (1907) Ñber die Bedingungen der Fuselölbildung und über ihren Zusammenhang mit dem Eiweißaufbau der Hefe. Ber. Dtsch. Chem. Ges. 40: 1027–1047.
Etschmann MMW, Bluemke W, Sell D, Schrader J (2002a) Biotechnological production of 2-phenylethanol. Appl. Microb. Biotechnol. 59: 1–8.
Etschmann M, Schrader J, Sell D (2002b) Microbial production of 2–phenylethanol by an integrated bioprocess. In: Proceedings of the 10th Weurman Symposium, June 24–28, Beaune, France (in press).
Fabre CE, Blanc PJ, Goma G (1997) Screening of yeasts producing 2-phenylethylalcohol. Biotechnol. Tech. 11: 253–252.
Fabre CE, Blanc PJ, Goma G (1998a) 2-Phenylethyl alcohol: an aroma profile. Perfum. Flavor. 23: 43–45.
Fabre CE, Blanc PJ, Goma G (1998b) Production of 2-phenylethyl alcohol by Kluyveromyces marxianus. Biotechnol. Prog. 14: 270–274.
Ingram LO, Buttke TM (1984) Effects of alcohols on microorganisms. Adv. Microb. Physiol. 25: 253–300.
Jiang J (1993) Identification of flavour volatile compounds produced by Kluyveromyces lactis. Biotechnol. Tech. 7: 863–866.
Lugay JC (1986) Biogeneration of aromas. An industrial perspective. In: Parliment T, Croteau R, eds. Biogeneration of Aromas. American Chemical Society, ACS Symposium Series 317, pp. 11–17.
Savina J-P, Kohler D, Brunerie P (1999) Method for extracting 2-phenylethanol. Patent US 005965780A.
Seward R, Willetts JM, Dinsdale MG, Lloyd D (1996) The effects of ethanol, hexan-1-ol, and 2-phenylethanol on cider yeast growth, viability, and energy status; synergistic inhibition. J. Inst. Brew. 102: 439–443.
Stark D (2001) Extractive bioconversion of 2-phenylethanol from Lphenylalanine by Saccharomyces cerevisiae. Dissertation, Lausanne, Switzerland: EPFL.
Stark D, Münch T, Sonnleitner B, Marison IW, von Stockar U (2002) Extractive bioconversion of 2-phenylethanol from Lphenylalanine by Saccharomyces cerevisiae. Biotechnol. Prog. 18: 514–523.
US Food and Drug Administration (2001) Code of federal regulations 21CFR101.22.
Vollbrecht D, Radler F (1973) Die Bildung höherer Alkohole bei Aminosäuremangelmutanten von Saccharomyces cerevisiae. I. Der Abbau von Aminosäuren zu höheren Alkoholen. Arch. Mikrobiol. 94: 351–358.
Weilnhammer C, Blass E (1994) Continuous fermentation with product recovery by in-situ extraction. Chem. Eng. Technol. 17: 365–373.
Wittman C, Hans M, Bluemke W (2002) Metabolic physiology of aroma-producing Kluyveromyces marxianus. Yeast 19: 1351–1363.
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Etschmann, M., Sell, D. & Schrader, J. Screening of yeasts for the production of the aroma compound 2-phenylethanol in a molasses-based medium. Biotechnology Letters 25, 531–536 (2003). https://doi.org/10.1023/A:1022890119847
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DOI: https://doi.org/10.1023/A:1022890119847