Article

Biotechnology Letters

, Volume 25, Issue 3, pp 219-222

(R)-Oxynitrilase-catalysed synthesis of chiral silicon-containing aliphatic (R)-ketone-cyanohydrins

  • Ning LiAffiliated withBiotechnology Department, South China University of Technology
  • , Min-Hua ZongAffiliated withBiotechnology Department, South China University of Technology Email author 
  • , Chen LiuAffiliated withBiotechnology Department, South China University of Technology
  • , Hua-Song PengAffiliated withBiotechnology Department, South China University of Technology
  • , Hua-Chang WuAffiliated withBiotechnology Department, South China University of Technology

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Abstract

Optically active 2-trimethylsilyl-2-hydroxyl-ethylcyanide was prepared by enzymatic enantioselective transcyanation of acetyltrimethylsilane with acetone cyanohydrin in a biphasic system at 35 °C and pH 5. (R)-Oxynitrilase from apple seed meal was the best among all the enzymes explored and diisopropyl ether was the most suitable organic phase. Acetyltrimethylsilane was a better substrate of the enzyme than its carbon analogue. The substrate conversion and product enantiomeric excess of 2-trimethylsilyl-2-hydroxyl-ethylcyanide were >99% and >99%, respectively.

asymmetric synthesis cyanohydrin organosilicon (R)-oxynitrilase 2-trimethylsilyl-2-hydroxyl-ethylcyanide