IFNγ and TNFα account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
In the present study, we found that murine peritoneal macrophages elicited by BCG or Listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of MHC class I antigens in B16 melanoma cells. A similar activity has previously been found in media conditioned by Corynebacterium parvum-elicited macrophages. Analysis by gel filtration chromatography of media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages revealed that the material responsible for the pro-clonogenic activity concentrated in chromatographic fractions corresponding to molecular weights (25 to 52 kDa) which are characteristic of certain cytokines. Thus, we challenged the various macrophage-conditioned media with polyclonal antibodies against IFNγand TNFα, and found that the macrophage pro-clonogenic activity was completely abolished in the presence of anti-IFNγantibodies, but only partially inhibited by anti-TNFαantibodies. This finding suggests a cooperative participation of the two cytokines to the pro-clonogenic activity of the media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages.
- Cecconi O, Calorini L, Mannini A et al. Enhancement of lung-colonizing potential of murine tumor cell lines co-cultivated with activated macrophages. Clin Exp Metastasis 1997; 15: 94–101. CrossRef
- Calorini L, Mannini A, Bianchini F et al. Biological properties associated with the enhanced lung-colonizing potential in a B16 murine melanoma line grown in a medium conditioned by syngeneic Corynebacterium parvum-elicited macrophages. Clin Exp Metastasis 1999; 17: 889–95. CrossRef
- Gattoni-Celli S, Calorini L, Simile MM et al. Modulation by MHC Class I antigens of the biology of melanoma cells. Non-immunological mechanisms. Melanoma Res 1993; 3: 285–9.
- Chen TR. In situ detection of Myeoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res 1977; 104: 255–62. CrossRef
- Havell EA, Spitalny GL. Two molecular weight species of murine gamma interferon. Virology 1983; 129: 508–13. CrossRef
- Wingfield P, Pain RH, Craig S. Tumor necrosis factor is a compact trimer. FEBS Lett 1987; 211: 179–84. CrossRef
- Billiau A. Interferon-γ: Biology and role in pathogenesis. Adv Immunol 1996; 62: 61–130. CrossRef
- Puddu P, Fantuzzi L, Borghi P et al. IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages. J. Immunol 1997; 159: 3490–7.
- Guillemard E, Geniteau-Legendre M, Kergot R et al. Simultaneous production of IFN-gamma, IFN-alpha/beta and nitric oxide in peritoneal macrophages from TDM-treated mice. J Biol Regul Homeost Agents 1998; 12: 106–11.
- Taniguchi K, Petersson M, Hoglund P et al. Interferon γ induces lung colonization by intravenously inoculated B16 melanoma cells in parallel with enhanced expression of class I major histocompatibility complex antigens. Proc Natl Acad Sci USA 1987; 84: 3405–9. CrossRef
- McMillan TJ, Rao J, Everett CA et al. Interferon-induced alterations in metastatic capacity, class-I antigen expression and natural killer cell sensitivity of melanoma cells. Int J Cancer 1987; 40: 659–63.
- Zoller M, Strubel A, Hammerling G et al. Interferon-gamma treatment of B16 melanoma cells: Opposing effects for non-adaptive and adaptive immune defense and its reflection by metastatic spread. Int J Cancer 1988; 41: 256–66.
- Ramani P, Balkwill FR. Enhanced metastases of a mouse carcinoma after in vitro treatment with murine interferon gamma. Int J Cancer 1987; 40: 830–4.
- Kelly SA, Gschmeissner S, East N et al. Enhancement of metastatic potential by γ-interferon. Cancer Res 1991; 51: 4020–7.
- Lollini PL, De Giovanni C, Nicoletti G et al. Enhancement of experimental metastatic ability by tumor necrosis factor-alpha alone or in combination with interferon-gamma. Clin Exp Metastasis 1990; 8: 215–24. CrossRef
- Piontek G, Taniguchi K, Ljunggren H et al. YAC-1 MHC class I variants reveal association between decreased NK sensitivity and increased H-2 expression after interferon treatment or in vivo passage. J Immunol 1985; 135: 4281–8.
- Taniguchi K, Karre K, Klein G. Lung colonization and metastasis by disseminated B16 melanoma cells: H-2 associated control at the level of the host and the tumor cells. Int J Cancer 1985; 36: 503–10.
- Kawano Y-I, Taniguchi K, Toshitani A et al. Synergistic defense system by cooperative natural effectors against metastasis of B16 melanoma cells in H-2-associated control: Different behaviour of H-2+ and H-2− cells in metastatic process. J Immunol 1986; 136: 4729–34.
- Carrel S, Hartmann F, Salvi S et al. Expression of type A and B tumor necrosis factor (TNF) receptors on melanoma cells can be regulated by dbe-AMP and IFNγ. Int J Cancer 1995; 62: 76–83.
- Saiki I, Maeda H, Murata J et al. Antimetastatic effect of endogenous tumor necrosis factor induced by the treatment of recombinant interferon gamma followed by an analogue (GLA-60) to synthetic lipid A subunit. Cancer Immunol Immunother 1989; 30: 151–157. CrossRef
- Schultz RM, Altom MG. Protective activity of recombinant murine tumor necrosis factor-alpha and interferon-gamma against experimental murine lung carcinoma metastases. J Interferon Res 1990; 10: 229–36.
- Ruegg C, Yilmaz A, Bieler G et al. Evidence for the involvement of endothelial cell integrin alphaVbeta3 in the disruption of the tumor vasculature induced by TNF and IFN-gamma. Nat Med 1998; 4: 408–14. CrossRef
- van Moorselaar RJA, Hendriks BT, van Stratum P et al. Synergistic antitumor effects of rat γ-interferon and human tumor necrosis factor α against androgen-dependent and-independent rat prostatic tumors. Cancer Res 1991; 51: 2329–34.
- Tracey KJ, Cerami A. Tumor necrosis factor: A pleiotropic cytokine and therapeutic target. Annu Rev Med 1994; 45: 491–503. CrossRef
- IFNγ and TNFα account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages
Clinical & Experimental Metastasis
Volume 19, Issue 3 , pp 259-264
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- F10-M3 cells
- Corynebacterium parvum-
- BCG- or Listeria monocytogenes-elicited macrophages
- MHC class I antigens
- lung colonization
- macrophage pro-clonogenic acitivity
- Industry Sectors
- Author Affiliations
- 1. Department of Experimental Pathology and Oncology, Radiation Biology Laboratory, University of Florence, Italy
- 2. Department of Clinical Physiopathology, Radiation Biology Laboratory, University of Florence, Italy