Article

Clinical & Experimental Metastasis

, Volume 19, Issue 3, pp 259-264

IFNγ and TNFα account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages

  • Lido CaloriniAffiliated withDepartment of Experimental Pathology and Oncology, Radiation Biology Laboratory, University of Florence
  • , Francesca BianchiniAffiliated withDepartment of Experimental Pathology and Oncology, Radiation Biology Laboratory, University of Florence
  • , Antonella ManniniAffiliated withDepartment of Experimental Pathology and Oncology, Radiation Biology Laboratory, University of Florence
  • , Gabriele MugnaiAffiliated withDepartment of Experimental Pathology and Oncology, Radiation Biology Laboratory, University of Florence
  • , Manuela BalziAffiliated withDepartment of Clinical Physiopathology, Radiation Biology Laboratory, University of Florence
  • , Aldo BeccioliniAffiliated withDepartment of Clinical Physiopathology, Radiation Biology Laboratory, University of Florence
  • , Salvatore RuggieriAffiliated withDepartment of Experimental Pathology and Oncology, Radiation Biology Laboratory, University of Florence

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Abstract

In the present study, we found that murine peritoneal macrophages elicited by BCG or Listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of MHC class I antigens in B16 melanoma cells. A similar activity has previously been found in media conditioned by Corynebacterium parvum-elicited macrophages. Analysis by gel filtration chromatography of media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages revealed that the material responsible for the pro-clonogenic activity concentrated in chromatographic fractions corresponding to molecular weights (25 to 52 kDa) which are characteristic of certain cytokines. Thus, we challenged the various macrophage-conditioned media with polyclonal antibodies against IFNγand TNFα, and found that the macrophage pro-clonogenic activity was completely abolished in the presence of anti-IFNγantibodies, but only partially inhibited by anti-TNFαantibodies. This finding suggests a cooperative participation of the two cytokines to the pro-clonogenic activity of the media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages.

F10-M3 cells Corynebacterium parvum- BCG- or Listeria monocytogenes-elicited macrophages MHC class I antigens lung colonization macrophage pro-clonogenic acitivity IFNγ TNFα