Clinical & Experimental Metastasis

, Volume 19, Issue 3, pp 259–264

IFNγ and TNFα account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages

Authors

  • Lido Calorini
    • Department of Experimental Pathology and Oncology, Radiation Biology LaboratoryUniversity of Florence
  • Francesca Bianchini
    • Department of Experimental Pathology and Oncology, Radiation Biology LaboratoryUniversity of Florence
  • Antonella Mannini
    • Department of Experimental Pathology and Oncology, Radiation Biology LaboratoryUniversity of Florence
  • Gabriele Mugnai
    • Department of Experimental Pathology and Oncology, Radiation Biology LaboratoryUniversity of Florence
  • Manuela Balzi
    • Department of Clinical Physiopathology, Radiation Biology LaboratoryUniversity of Florence
  • Aldo Becciolini
    • Department of Clinical Physiopathology, Radiation Biology LaboratoryUniversity of Florence
  • Salvatore Ruggieri
    • Department of Experimental Pathology and Oncology, Radiation Biology LaboratoryUniversity of Florence
Article

DOI: 10.1023/A:1015583322354

Cite this article as:
Calorini, L., Bianchini, F., Mannini, A. et al. Clin Exp Metastasis (2002) 19: 259. doi:10.1023/A:1015583322354

Abstract

In the present study, we found that murine peritoneal macrophages elicited by BCG or Listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of MHC class I antigens in B16 melanoma cells. A similar activity has previously been found in media conditioned by Corynebacterium parvum-elicited macrophages. Analysis by gel filtration chromatography of media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages revealed that the material responsible for the pro-clonogenic activity concentrated in chromatographic fractions corresponding to molecular weights (25 to 52 kDa) which are characteristic of certain cytokines. Thus, we challenged the various macrophage-conditioned media with polyclonal antibodies against IFNγand TNFα, and found that the macrophage pro-clonogenic activity was completely abolished in the presence of anti-IFNγantibodies, but only partially inhibited by anti-TNFαantibodies. This finding suggests a cooperative participation of the two cytokines to the pro-clonogenic activity of the media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages.

F10-M3 cellsCorynebacterium parvum-BCG- or Listeria monocytogenes-elicited macrophagesMHC class I antigenslung colonizationmacrophage pro-clonogenic acitivityIFNγTNFα

Copyright information

© Kluwer Academic Publishers 2002