Pharmaceutical Research

, Volume 18, Issue 11, pp 1593–1599

On the Release of Proteins from Degrading Dextran Methacrylate Hydrogels and the Correlation with the Rheologic Properties of the Hydrogels

Authors

  • Tom Meyvis
    • Laboratory of General Biochemistry and Physical PharmacyGhent University
  • Stefaan De Smedt
    • Laboratory of General Biochemistry and Physical PharmacyGhent University
  • Barbara Stubbe
    • Laboratory of General Biochemistry and Physical PharmacyGhent University
  • Wim Hennink
    • Department of Pharmaceutics, Utrecht Institute for Pharmaceutical SciencesUniversity of Utrecht
  • Joseph Demeester
    • Laboratory of General Biochemistry and Physical PharmacyGhent University
Article

DOI: 10.1023/A:1013038716373

Cite this article as:
Meyvis, T., De Smedt, S., Stubbe, B. et al. Pharm Res (2001) 18: 1593. doi:10.1023/A:1013038716373

Abstract

Purpose. To study the release of macromolecules of different sizes (bovine serum albumin, immunoglobulin G) from degrading (addition of dextranase) dextran methacrylate (dex-MA) hydrogels and to correlate the release with the evolution of the rehologic properties of the hydrogels during degradation.

Methods. The size of the macromolecules, the degree of substitution (i.e., number of methacrylates per 100 glycopyranose residues) of the dex-MA and the dextranase concentration in the hydrogels was varied. The rheologic properties were measured with a controlled stress rheometer.

Results. The release from dex-MA hydrogels without dextranase was very small [7-20% (time frame up to 180 days)] showing that most of the molecules were entrapped within the hydrogel network. The release from degrading dex-MA hydrogels followed zero-order kinetics for all molecules during a substantial period of the release. This was explained by a liberation and an increasing diffusivity of the proteins in the course of the degradation. The total amount released and the release rates could be well correlated with the rheologically observed degradation rates.

Conclusions. It was shown that rheology can be a useful tool to help explain the release from degrading hydrogels.

hydrogels controlled release rheology proteins dextran methacrylate

Copyright information

© Plenum Publishing Corporation 2001