Biologia Plantarum

, Volume 44, Issue 3, pp 417–421

An Antifungal Protein Purified from Pearl Millet Seeds Shows Sequence Homology to Lipid Transfer Proteins

Authors

  • R. Velazhahan
    • Department of Plant PathologyTamil Nadu Agricultural University
  • R. Radhajeyalakshmi
    • Department of Plant PathologyTamil Nadu Agricultural University
  • R. Thangavelu
    • Department of Plant PathologyTamil Nadu Agricultural University
  • S. Muthukrishnan
    • Department of BiochemistryKansas State University
Article

DOI: 10.1023/A:1012463315579

Cite this article as:
Velazhahan, R., Radhajeyalakshmi, R., Thangavelu, R. et al. Biologia Plantarum (2001) 44: 417. doi:10.1023/A:1012463315579

Abstract

In the course of a search for antifungal proteins from plant seeds, we observed inhibition of mycelial growth of Trichoderma viride with extracts of pearl millet. We have identified several proteins with antifungal properties in the seeds of pearl millet. One of these proteins has been purified to homogeneity and characterized. The purified protein has a molecular mass of 25 kDa. The N-terminal sequence of the protein (25 residues) shows homology to non-specific lipid transfer proteins (LTPs) of cotton, wheat and barley. The purified LTP inhibited mycelial growth of T. viride and the rice sheath blight fungus, Rhizoctonia solani in vitro.

pathogenesis-related proteinPennisetum glaucumRhizoctonia solaniTrichoderma viride

Copyright information

© Kluwer Academic Publishers 2001