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A simple method for efficient separation and purification of c-phycocyanin and allophycocyanin from Spirulina platensis

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Biotechnology Techniques

Abstract

c-Phycocyanin and allophycocyanin were separated and purified from Spirulina platensis by precipitation with ammonium sulphate, ion exchange chromatography and gel filtration chromatography. Pure c-phycocyanin and allophycocyanin were finally obtained with an A620/A280value of 5.06 and an A655/A280 value of 5.34, respectively.

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Authors and Affiliations

  1. Light Industry Department, Guizhou University of Technology, Guiyang, China

    Yi-Ming Zhang

  2. Department of Botany, the University of Hong Kong, Pokfulam Road, Hong Kong

    Feng Chen

Authors
  1. Yi-Ming Zhang
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  2. Feng Chen
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Zhang, YM., Chen, F. A simple method for efficient separation and purification of c-phycocyanin and allophycocyanin from Spirulina platensis . Biotechnology Techniques 13, 601–603 (1999). https://doi.org/10.1023/A:1008914405302

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  • DOI: https://doi.org/10.1023/A:1008914405302

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