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Effect of oxygen supply on biomass, organic acids and vitamin B12 production by Propionibacterium shermanii

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Abstract

Propionibacterium shermanii CDB 10015 was able to grow at different volumetric oxygen transfer coefficients (KLa) of 10, 22, 53h−1. These results demonstrate that this bacterium, known as anaerobic, is able to grow well under aerobic conditions. The cell biomass increased from 7.9 in anaerobic conditions to 18.3g/l at KLa 53h−1, increasing also the cell yield from 0.3 to 0.7g/g. The organic acid production pattern also changed with aeration. The acetic: propionic acid ratio increased from 0.38 in anaerobiosis to 6.25 at KLa 53h−1. The vitamin B12 production decreased from 3.1mg/l in anaerobiosis to 0.5mg/l at KLa 53h−1.

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References

  • Berry, E.C. & Bullerman, L.B. 1966 Use of Cheese Whey for Vitamin B12 Production. Applied Microbiology 14, 356–357.

    Google Scholar 

  • Blanc, P. & Goma, G. 1987 Propionic acid fermentation improvement of performances by coupling continuous fermentation and ultrafiltration. Bioprocess Engineering 2, 137–139.

    Google Scholar 

  • Blanc, P. & Goma, G. 1989 Propionic aicd and biomass production using continuous ultrafiltration fermentation of whey. Biotechnology Letters 11, 189–194.

    Google Scholar 

  • Cummins, C.S. & Johnson, J.L. 1992 The Genus Propionibacterium. In The Prokaryotes, ed Balow, A. Vol. 1. pp 834–849. New York, Berlin, Heidelberg: Springer Verlag. ISBN 3–54097258–7.

    Google Scholar 

  • de Vries, W., van Wijck-Kapteijn, W.M.C. & Stouthamer, A.H. 1972 Influence of oxygen on growth, cytochrome synthese and fermentation pattern in propionic acid Bacteria. Journal of General Microbiology 71, 515–524.

    Google Scholar 

  • de Vries, W., van Wijck-Kapteijn, W.M.C. & Stouthamer, A.H. 1973 Generation of ATP during cytochrome-linked anaerobic electron transport in propionic acid bacteria. Journal of General Microbiology 76, 31–41.

    Google Scholar 

  • Fischer, R.A. 1953 Rapid spectrophotometric determination of vitamin B12 in microbial material. Agricultural and Food Chemistry 1, 951–953.

    Google Scholar 

  • Florent, J. 1986 Vitamins. In Biotechnology, eds Rehm, H.J. & Reed, R. Vol. 4, pp 119–158, Weinheim: VCH.ISBN 3–527–25764–0.

    Google Scholar 

  • Flores-Galagarza, R.A., Glatz, B.A., Bern, C.J. & Fossen, L.D. 1985 Preservation of high moisture corn by microbial fermentation. Journal of Food Protection 48, 407–411.

    Google Scholar 

  • Gottschalk, G. 1986 Bacterial metabolism. 2nd ed. pp 244–246. New York, Berlin, Heidelberg: Springer Verlag ISBN 3–54096153–4.

    Google Scholar 

  • Mantere-Alhonen, S. & Mäkinen, E. 1987 A new type of sour milk with Propionibacteria. Meijeritieteellinen Aikakauskirja 45, 49–61.

    Google Scholar 

  • Miles-Ames 1983 Silage production from grass or grain crops European patent: EP-71858; 16.02.83.

  • Molwitz, M., Silva, S.S., Ribeiro, J.D., Roberto, I.C., Felipe, M.G.A., Prata, A.M. & Mancilha, I.M. 1996 Aspects of the cell growth of Candida guilliermondii in sugar cane bagasse hydrolysate. Zeitschrift für Naturforschung 51c, 404–408.

    Google Scholar 

  • Nakano, K., Kataoka, H. & Matsumura, M. 1996 High density culture of Propionibacterium freundenreichii coupled with a propionic acid removal system with activated charcoal. Journal of Fermentation and Bioengineering 81, 37–41.

    Google Scholar 

  • Pirt, S.J. 1975 Principles of Microbe and Cell Cultivation. Oxford: Blackwell Scientific Publications, chapters 9–11. ISBN 0–63208150–3.

    Google Scholar 

  • Playne, M.J. 1985 Propionic and butyric acid. In Comprehensive Biotechnology. ed Moo-Young, M., vol. 3, pp 731–759, New York: Pergamon Press. ISBN 0–08032511–4.

    Google Scholar 

  • Quesada-Chanto, A., Afschar, A.S., Wagner, F. 1994a Microbial production of propionic acid and vitamin B12 using molasses or sugar. Applied Microbiology and Biotechnology 41, 378–383.

    Google Scholar 

  • Quesada-Chanto, A., Afschar, A.S., Wagner, F. 1994b Optimization of a Propionibacterium acidipropionici continuous culture utilizing sucrose. Applied Microbiology and Biotechnology 42, 16–21.

    Google Scholar 

  • Quesada-Chanto, A., Schroeder, A., Schmidt-Meyer, A., López, J.A., Silveira, M.M. & Jonas, R. 1997 Organic acids production by Propionibacterium shermanii: Effect of pH, temperature, and vitamin-nitrogen source. Zeitschrift für Naturforschung 52c, 193–196.

    Google Scholar 

  • Rehm, H.J. 1980 Industrielle Mikrobiologie. Berlin, Heidelberg, New York: Springer Verlag. ISBN 3–540–09642–6.

    Google Scholar 

  • Santana-Castillo, L., Pérez-Mendoza, J.L., & García-Hernández, P. 1985 Production of vitamin B12 by fermentation of an industrial waste-product of mexican lime. Development in Industrial Microbiology 26, 796–780.

    Google Scholar 

  • Schwartz, A.C. 1973 Anaerobiosis and oxygen consumption of some strains of Propionibacterium and a modified method of comparing the oxygen sensitivity of various anaerobes. Zeitschrift für Allgemeine Mikrobiologie 13, 681–691.

    Google Scholar 

  • Schwartz, A.C. & Sporkenbach, J. 1975 The Electron transport system of the anaerobic Propionibacterium shermanii. Cytochrome and inhibitor studies. Archives of Microbiology 102, 261–273.

    Google Scholar 

  • Schlegel, H.G. 1992 Allgemeine Mikrobiologie. 7th ed., pp 304–307, Stuttgart, New York: Georg Thieme Verlag. ISBN 3–13–444607–3.

    Google Scholar 

  • Valio 1994 New Lactobacillus casei ssp. rhamnosus strain. European patent: EP 575780; 05.01.94.

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Quesada-Chanto, A., Schmid-Meyer, A., Schroeder, A. et al. Effect of oxygen supply on biomass, organic acids and vitamin B12 production by Propionibacterium shermanii. World Journal of Microbiology and Biotechnology 14, 843–846 (1998). https://doi.org/10.1023/A:1008868907251

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  • DOI: https://doi.org/10.1023/A:1008868907251

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