Abstract
Spectral density mapping provides direct access to protein dynamics with no assumptions as to the nature of the molecule or its dynamic behaviour. Reduced spectral density mapping characterises a protein's motions at a lower experimental burden, assuming that the spectral density function J(ω) is flat around ωH. This introduces little error for 15N relaxation data but is less valid for 13C studies, perturbing J(ωC) considerably to an extent that depends on the nature of the molecule’s motions. We propose the fitting of spectral density at high frequencies to a single Lorentzian and show that the true values of the spectral density lie between those determined by the two approximations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abragam, A. (1961) Principles of Nuclear Magnetism, Oxford University Press, Oxford.
Dayie, K.T., Wagner, G. and Lefèvre, J.-F. (1996) In Dynamics and the Problem of Recognition in Biological Macromolecules (Eds., Jardetzky, O. and Lefèvre, J.-F.) Plenum Press, New York, NY, pp. 139–162.
Farrow, N.A., Zhang, O., Szabo, A., Torchia, D.A. and Kay, L.E. (1995a) J. Biomol. NMR, 6, 153–162.
Farrow, N.A., Zhang, O., Forman-Kay, J.D. and Kay, L.E. (1995b) Biochemistry, 34, 868–878.
Goffe, W.L., Ferrier, G.D. and Rogers, J. (1994) J. Econometrics, 60, 65–99.
Ishima, R. and Nagayama, K. (1995a) J. Magn. Reson., B108, 73–76.
Ishima, R. and Nagayama, K. (1995b) Biochemistry, 34, 3162–3171.
Kirkpatrick, S., Gelatt Jr., C.D. and Vecchi, M.P. (1983) Science, 220, 671–680.
Kördel, J., Skelton, N.J., Akke, M., Palmer, A.G. and Chazin, W.J. (1992) Biochemistry, 31, 4856–4866.
Korzhnev, D.M., Orekhov, V.Y. and Arseniev, A.S. (1997) J. Magn. Reson., 127, 184–191.
Lefèvre, J.-F., Dayie, K.T., Peng, J.W. and Wagner, G. (1996) Biochemistry, 35, 2674–2686.
Lipari, G. and Szabo, A. (1982a) J. Am. Chem. Soc., 104, 4546–4559.
Lipari, G. and Szabo, A. (1982b) J. Am. Chem. Soc., 104, 4559–4570.
Luginbühl, P., Pervushin, K.V., Iwai, H. and Wüthrich, K. (1997) Biochemistry, 36, 7305–7312.
Mer, G., Dejaegere, A., Stote, R., Kieffer, B. and Lefèvre, J.-F. (1996) J. Chem. Phys., 100, 2667–2674.
Palmer III, A.G. (1997) Curr. Opin. Struct. Biol., 7, 732–737.
Peng, J.W. and Wagner, G. (1992a) J. Magn. Reson., 98, 308–332.
Peng, J.W. and Wagner, G. (1992b) Biochemistry, 31, 8571–8586.
Peng, J.W. and Wagner, G. (1995) Biochemistry, 34, 16733–16752.
van Heijenoort, C., Penin, F. and Guittet, E. (1998) Biochemistry, 37, 5060–5073.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Atkinson, R.A., Lefèvre, JF. Reduced spectral density mapping for proteins: Validity for studies of 13C relaxation. J Biomol NMR 13, 83–88 (1999). https://doi.org/10.1023/A:1008323420405
Issue Date:
DOI: https://doi.org/10.1023/A:1008323420405