Journal of Applied Phycology

, Volume 12, Issue 3, pp 249–255

Nannochloropsis (Eustigmatophyceae) as source of commercially valuable pigments

Authors

  • Luis M. Lubián
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
  • Olimpio Montero
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
  • Ignacio Moreno-Garrido
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
  • I. Emma Huertas
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
  • Cristina Sobrino
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
  • Manuel González-del Valle
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
  • Griselda Parés
    • Instituto de Ciencias Marinas de Andalucía (ICMAN, CSIC)
Article

DOI: 10.1023/A:1008170915932

Cite this article as:
Lubián, L.M., Montero, O., Moreno-Garrido, I. et al. Journal of Applied Phycology (2000) 12: 249. doi:10.1023/A:1008170915932

Abstract

Pigment composition and its variation with culture agewere analyzed in six strains of Nannochloropsis(Eustigmatophyceae). The capacity for accumulationof the ketocarotenoids astaxanthin and canthaxanthinwas higher in N. salina and N. gaditanathan in the other strains studied here. Theinfluence of salinity (15 to 100 practical units) onpigment production was studied in N. gaditana,where a defined pattern of variation could not befound apart from a notable increase in zeaxanthin at100‰. In cultures grown in a photobioreactor and athigh cell densities of about 109 cells mL-1,pigment production reached: 350 mg L-1 forchlorophyll a, 50 mg L-1 for violaxanthin,5 mg L-1 for canthaxanthin, 3 mg L-1 forastaxanthin. The highest contents of canthaxanthin andastaxanthin obtained in experiments with N.gaditana were 19.4 and 14.6 ng pigment (106cells)-1, respectively, which accounts for 0.7%dry weight. By means of xanthophyll cycle inductionthrough exposure of cells to high irradiance and at40 °C, conversion of violaxanthin intozeaxanthin may attain up to 70% of the violaxanthincontent, which corresponds to 0.6% dry weight. Theresults indicate that interest in Nannochloropsis as a source of valuable pigments isnot related to its capacity for single pigmentaccumulation, but the availability of a range ofpigments such as chlorophyll a, zeaxanthin,canthaxanthin and astaxanthin, each with highproduction levels.

pigments ketocarotenoids xanthophyll cycle microalgae culture Nannochloropsis Eustigmatophyceae HPLC

Copyright information

© Kluwer Academic Publishers 2000