Article

Cytotechnology

, Volume 28, Issue 1, pp 163-175

Mammalian cell retention devices for stirred perfusion bioreactors

  • Steven M. WoodsideAffiliated withBiotechnology Laboratory and Department of Chemical and Bio- Resource Engineering, University of British Columbia
  • , Bruce D. BowenAffiliated withBiotechnology Laboratory and Department of Chemical and Bio- Resource Engineering, University of British Columbia
  • , James M. PiretAffiliated withBiotechnology Laboratory and Department of Chemical and Bio- Resource Engineering, University of British Columbia Email author 

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Abstract

Within the spectrum of current applications for cell culture technologies, efficient large-scale mammalian cell production processes are typically carried out in stirred fed-batch or perfusion bioreactors. The specific aspects of each individual process that can be considered when determining the method of choice are presented. A major challenge for perfusion reactor design and operation is the reliability of the cell retention device. Current retention systems include cross-flow membrane filters, spin-filters, inclined settlers, continuous centrifuges and ultrasonic separators. The relative merits and limitations of these technologies for cell retention and their suitability for large-scale perfusion are discussed.

centrifuge cross-flow filter review sedimentation spin-filter ultrasonic cell retention