Cytotechnology

, Volume 28, Issue 1, pp 163–175

Mammalian cell retention devices for stirred perfusion bioreactors

Authors

  • Steven M. Woodside
    • Biotechnology Laboratory and Department of Chemical and Bio- Resource EngineeringUniversity of British Columbia
  • Bruce D. Bowen
    • Biotechnology Laboratory and Department of Chemical and Bio- Resource EngineeringUniversity of British Columbia
    • Biotechnology Laboratory and Department of Chemical and Bio- Resource EngineeringUniversity of British Columbia
Article

DOI: 10.1023/A:1008050202561

Cite this article as:
Woodside, S.M., Bowen, B.D. & Piret, J.M. Cytotechnology (1998) 28: 163. doi:10.1023/A:1008050202561

Abstract

Within the spectrum of current applications for cell culture technologies, efficient large-scale mammalian cell production processes are typically carried out in stirred fed-batch or perfusion bioreactors. The specific aspects of each individual process that can be considered when determining the method of choice are presented. A major challenge for perfusion reactor design and operation is the reliability of the cell retention device. Current retention systems include cross-flow membrane filters, spin-filters, inclined settlers, continuous centrifuges and ultrasonic separators. The relative merits and limitations of these technologies for cell retention and their suitability for large-scale perfusion are discussed.

centrifugecross-flow filterreviewsedimentationspin-filterultrasonic cell retention

Copyright information

© Kluwer Academic Publishers 1998