Maintenance of steelhead trout (Oncorhynchus mykiss) sperm at different in vitro oxygen tensions alters ATP levels and cell functional characteristics
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Adenosine triphosphate (ATP) levels in sperm from steelhead trout (Oncorhynchus mykiss) were found to be 12.0 ± 1.4 pmol ATP per 106 sperm cells. Sperm were stored at 0–2 °C for up to 72 h under 100 and 21% O2, and 100% N2. The sperm ATP content of samples maintained under 100 and 21% O2 both decreased to about 70 and 50% of initial values after 24 and 72 h, respectively. ATP levels of sperm stored under 100% N2 decreased to 20% of initial values after only 4 h and to less than 10% of initial values after 24 h; they remained unchanged throughout the following 48 h. These low levels, however, were reversible. Following 4 h incubation under 100% N2, sperm ATP levels rose upon exposure to and maintenance under 100% O2, increasing to 50% of initial values after a total of 72 h. ATP levels in sperm maintained with cyanide for 4 h decreased to less than 5% of initial values. The magnitude of the change in sperm ATP levels after 24 h in samples maintained under 100% O2 correlated positively with the initial sperm ATP levels. Sperm ATP levels, motility, and fertility were all correlated positively in a subset of samples examined. The initial percent of living sperm (sperm viability) was 97.5 ± 0.5% and was unaltered through 24 h under all O2 tensions. Sperm viability remained unchanged through 72 h under 21% O2 and decreased by 10 and 30% by 72 h under 100% O2 and 100% N2, respectively. Therefore, reductions in sperm ATP levels could not be attributed to cell death as viability decreased only modestly over these durations. Spermatocrit values were unaltered throughout the 72 h incubation for samples maintained under 100 and 21% O2, however, a 95% increase in spermatocrit occurred after 72 h in samples maintained under 100% N2. These data indicate that intracellular ATP in steelhead trout sperm can be modulated by ambient oxygen without significant loss of viability and that this ATP has a probable role in cell motility and fertility, and sperm packing and cell flexibility.
- Benau, J. and Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res. 3: 247–257.
- Bencic, D.C., Yates, T.J., and Ingermann, R.L. 1997. Ecto-ATPase activity of vertebrate blood cells. Physiol. Zool. 70: 621–630.
- Bencic, D.C., Krisfalusi, M., Cloud, J.G., and Ingermann, R.L. 1999a. ATP levels of chinook salmon (Oncorhynchus tshawytscha) sperm following in vitro exposure to various oxygen tensions. Fish Physiol. Biochem. 20: 389–397.
- Bencic, D.C., Krisfalusi, M., Cloud, J.G., and Ingermann, R.L. 1999b. Short-term storage of salmonid sperm in air versus oxygen. N. Am. J. Aquacult. (In press).
- Billard, R., Petit, J., Jalabert, B. and Szollosi, D. 1974. Artificial insemination in trout using a sperm diluent. In: The Early Life History of Fish. pp. 715–723. Edited by J.H.S. Blaxter. Springer-Verlag, New York.
- Billard, R. 1981. Short-term preservation of sperm under oxygen atmosphere in rainbow trout (Salmo gairdneri). Aquaculture 23: 287–293.
- Birchmeier, W. and Singer, S.J. 1977. On the mechanism of ATP-induced shape changes in human erythrocyte membranes. I. The role of ATP. J. Cell Biol. 73: 647–659.
- Büyükhatipoglu, S., and Holtz, W. 1978. Preservation of trout sperm in liquid or frozen state. Aquaculture 14: 49–56.
- Chiocchia, G. and Motais, R. 1989. Effect of catecholamines on deformability of red cells from trout: relative roles of cyclic AMP and cell volume. J. Physiol. 412: 321–332.
- Christen, R., Gatti, J-L., and Billard, R. 1987. Trout sperm motility. The transient movement of trout sperm is related to changes in the concentration of ATP following the activation of the flagellar movement. Eur. J. Biochem. 166: 667–671.
- Ciereszko, A., and Dabrowski, K. 1994. Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage. Fish Physiol. Biochem. 12: 357–367.
- Cosson, M.-P., Cosson, J. and Billard, R. 1991. Synchronous triggering of trout sperm is followed by an inevitable set sequence of movement parameters whatever the incubation medium. Cell Mot. Cytoskeleton 20: 555–68.
- de Lamirande, E. and Gagnon, C. 1992a. Reactive oxygen species and human spermatozoa: I. Effects on the motility of intact spermatozoa and on sperm axonemes. J. Androl. 13: 368–378.
- de Lamirande, E. and Gagnon, C. 1992b. Reactive oxygen species and human spermatozoa: II. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J. Androl. 13: 379–386.
- Ferrell, J.E., Jr. and Huestis, W.H. 1984. Phosphoinositide metabolism and the morphology of human erythrocytes. J. Cell Biol. 98: 1992–1998.
- Holtz, W., Büyükhatipoglu, S., Stoss, J., Oldigs, B., and Langholz, H.-J. 1979. Preservation of trout spermatozoa for varying periods. In: Advances in Aquaculture. pp. 141–142. Edited by T.V.R. Pillay and W.A. Dill. Fishing New Books Ltd., Farnham, Surrey, England.
- Hughes, G.M. and Kikuchi, Y. 1984. Effects of in vivo and in vitro changes in Po2 on the deformability of red blood cells of rainbow trout (Salmo gairdneri R.). J. Exp. Biol. 111: 253–257.
- Lahnsteiner, F., Patzner, R.A., and Weismann, T. 1993. Energy resources of spermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces, Teleostei). Reprod. Nutr. Dev. 33: 349–360.
- Moccia, R.D. and Munkittrick, K.R. 1987. Relationship between the fertilization of rainbow trout (Salmo gairdneri) eggs and the motility of spermatozoa. Theriogenology 27: 679–688.
- Munkittrick, K.R. and Moccia, R.D. 1987. Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constituents. Aquaculture 64: 147–156.
- Nikinmaa, M. and Huestis, W.H. 1984. Shape changes in goose erythrocytes. Biochim. Biophys. Acta 773: 317–320.
- Robitaille, P-M.L., Mumford, K.G., and Brown, G.G. 1987. 31 P nuclear magnetic resonance study of trout spermatozoa at rest, after motility, and during short-term storage. Biochem. Cell. Biol. 65: 474–485.
- Sheetz, M.P. and Singer, S.J. 1977. On the mechanism of ATP-induced shape changes in human erythrocyte membranes. I. The role of the spectrin complex. J. Cell Biol. 73: 633–646.
- Stoss, J., Büyükhatipoglu, S., and Holtz, W. 1978. Short-term and cryopreservation of rainbow trout (Salmo gairdneri Richardson) sperm. Ann. Biol. Anim. Biochim. Biophys. 18: 1077–1082.
- Stoss, J. 1983. Fish gamete preservation and spermatozoan physiology. In: Fish Physiology. pp. 305–350. Edited by W. Hoar, D. Randall, and E. Donaldson. Academic Press, NY.
- Terner, C. and Korsh, G. 1963a. The oxidative metabolism of pyruvate, acetate and glucose in isolated fish spermatozoa. J. Cell. Comp. Physiol. 62: 243–249.
- Terner, C. and Korsh, G. 1963b. The biosynthesis of fatty acids, of glycerides and phosphatides by isolated spermatozoa of the rainbow trout. J. Cell. Comp. Physiol. 62: 251–255.
- Terner, C. 1986. Evaluation of salmonid sperm motility for cryopreservation. Progr. Fish-Cult. 48: 230–232.
- Wittenberg, B.A. and Wittenberg, J.B. 1985. Oxygen pressure gradients in isolated cardiac myocytes. J. Biol. Chem. 260: 6548–6554.
- Maintenance of steelhead trout (Oncorhynchus mykiss) sperm at different in vitro oxygen tensions alters ATP levels and cell functional characteristics
Fish Physiology and Biochemistry
Volume 21, Issue 3 , pp 193-200
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- adenosine triphosphate