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Immunocytochemical localization of d-amino acid oxidase in rat brain

  • Published:
Journal of Neurocytology

Abstract

d-amino acid oxidase (d-AAO) is a peroxisomal flavoenzyme, the physiological substrate and the precise function of which are still unclear. We have investigated D-AAO distribution in rat brain, by immunocytochemistry, with an affinity-purified polyclonal antibody. Immunoreactivity occurred in both neuronal and glial cells, albeit at different densities. Glial immunostaning was strongest in the caudal brainstem and cerebellar cortex, particularly in astrocytes, Golgi-Bergmann glia, and tanycytes. Hindbrain neurons were generally more immunoreactive than those in the forebrain. Immunopositive forebrain cell populations included mitral cells in the olfactory bulb, cortical and hippocampal neurons, ventral pallidum, and septal, reticular thalamic, and paraventricular hypothalamic nuclei. Within the positive regions, not all the neuronal populations were equally immunoreactive; for example, in the thalamus, only the reticular and anterodorsal nuclei showed intense labelling. In the hindbrain, immunopositivity was virtually ubiquitous, and was especially strong in the reticular formation, pontine, ventral and dorsal cochlear, vestibular, cranial motor nuclei, deep cerebellar nuclei, and the cerebellar cortex, especially in Golgi and Purkinje cells.

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Authors and Affiliations

  1. Dipartimento di Biologia, Università Roma Tre, Roma, 00146, Italy

    Sandra Moreno

  2. Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma La Sapienza, Roma, 00185, Italy

    Roberta Nardacci

  3. Dipartimento di Biologia di Base ed Applicata, Università dell'Aquila, Coppito, 67010, L'Aquila, Italy

    AnnaMaria Cimini & Maria Paola Cer`

Authors
  1. Sandra Moreno
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  2. Roberta Nardacci
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  3. AnnaMaria Cimini
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  4. Maria Paola Cer`
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Moreno, S., Nardacci, R., Cimini, A. et al. Immunocytochemical localization of d-amino acid oxidase in rat brain. J Neurocytol 28, 169–185 (1999). https://doi.org/10.1023/A:1007064504007

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