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Azo dye decolorization with a mutant Escherichia coli strain

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Abstract

A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h−1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l−1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.

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References

  • Banat IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textile-dye-containting effluents: A review. Biores.Technol. 58: 217–227.

    Google Scholar 

  • Carliell CM, Barclay SJ, Naidoo N, Buckley CA, Mulholland DA, Senior E (1995) Microbial decolourization of a reactive azo dye under anaerobic conditions. Water SA 21: 61–69.

    Google Scholar 

  • Chung K-T, Stevens SE Jr (1993) Degradation of azo dyes by environmental microorganisms and helminths. Environ.Toxicol.Chem. 12: 2121–2132.

    Google Scholar 

  • Coughlin MF, Kinkle BK, Tepper A, Bishop PL (1997) Characterization of aerobic azo dye-degrading bacteria and their activity in biofilms. Wat.Sci.Technol. 36: 215–220.

    Google Scholar 

  • Flores ER, Luijten M, Donlon BA, Lettinga G, Field JA (1997) Complete biodegradation of the azo dye azodisalicylate under anaerobic conditions. Environ.Sci.Technol. 31: 2098–2103.

    Google Scholar 

  • Ghosh DK, Ghosh S, Sadhukhan P, Mandal A, Chaudhuri J (1993) Purification of two azoreductases from Escherichia coli K12. Ind.J.Exp.Biol. 31: 951–954.

    Google Scholar 

  • Glenn JK, Gold MH (1983) Decolorization of several polymeric dyes by the lignin-degrading Basidiomycete Phanerochaete chrysosporium. Appl.Environ.Microbiol. 45: 1741–1747.

    Google Scholar 

  • Hashimoto-Gotoh T, Tsujimura A, Kuriyama K-Y, Matsuda S (1993) Construction and characterization of new host-vector systems for the enforcement-cloning method. Gene 137: 211–216.

    PubMed  Google Scholar 

  • Haug W, Schmidt A, Nortemann B, Hempel DC, Stolz A, Knackmuss H-J (1991) Mineralization of the sulfonated azo dye mordant yellow 3 by 6-aminonaphthalene-2-sulfonate-degrading bacterial consortium. Appl.Environ.Microbiol. 57: 3144–3149.

    PubMed  Google Scholar 

  • Heiss GS, Gowan B, Dabbs ER (1992) Cloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes. FEMS Microbiol.Lett. 99: 221–226.

    Google Scholar 

  • Hu TL (1994) Decolourization of reactive azo dyes by transformation with Pseudomonas luteola. Biores.Technol. 49: 47–51.

    Google Scholar 

  • Michaels GB, Lewis DL (1986) Microbial transformation rates of azo and triphenylmethane dyes. Environ.Toxicol.Chem. 5: 161–166.

    Google Scholar 

  • Nigam P, Marchant R (1995) Selection of a substratum for composing biofilm system of a textile-effluent decolorizing bacteria. Biotechol.Lett. 17: 993–996.

    Google Scholar 

  • Rafii F, Coleman T (1999) Cloning and expression in Escherichia coli of an azoreductase gene from Clostridium perfringens and comparison with azoreductase genes from other bacteria. J.Basic Microbiol. 39: 29–35.

    PubMed  Google Scholar 

  • Seshadri S, Bishop PL, Agha AM (1994) Anaerobic/aerobic treatment of selected azo dyes in wastewater. Waste Manage. 14: 127–137.

    Google Scholar 

  • Yang F, Yu J (1996) Development of a bioreactor system using an immobilized white rot fungus for decolourization, Part II: Continuous decolourization tests. Bioprocess Eng. 16: 9–11.

    Google Scholar 

  • Zhang F-M, Knapp JS, Tapley KN (1999) Development of bioreactor systems for decolorization of orange II using white rot fungus. Enzyme Microbiol.Technol. 24: 48–53.

    Google Scholar 

  • Zimmermann T, Kulla HG, Leisinger T (1982) Properties of purified orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46. Eur.J.Biochem. 129: 197–203.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Feng Chia University, Taichung, Taiwan, R.O.C.

    Jo-Shu Chang, Tai-Shin Kuo, Tai-Shin Kuo, Yun-Peng Chao, Yun-Peng Chao, Jin-Yen Ho, Jin-Yen Ho, Ping-Jei Lin & Ping-Jei Lin

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  1. Jo-Shu Chang
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  2. Tai-Shin Kuo
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  3. Yun-Peng Chao
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  4. Jin-Yen Ho
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  5. Ping-Jei Lin
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Chang, JS., Kuo, TS., Chao, YP. et al. Azo dye decolorization with a mutant Escherichia coli strain. Biotechnology Letters 22, 807–812 (2000). https://doi.org/10.1023/A:1005624707777

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