Skip to main content
Log in

From Bacteriology to Biochemistry: Albert Jan Kluyver and Chester Werkman at Iowa State

  • Published:
Journal of the History of Biology Aims and scope Submit manuscript

Abstract

This essay explores connections between bacteriology and the disciplinary evolution of biochemistry in this country during the 1930s. Many features of intermediary metabolism, a central component of biochemistry, originated as attempts to answer fundamental bacteriological questions. Thus, many bacteriologists altered their research programs to answer these questions. In so doing they changed their disciplinary focus from bacteriology to biochemistry. Chester Hamlin Werkman's (1893–1962) Iowa State career illustrates the research perspective that many bacteriologists adopted. As a junior faculty member in the Bacteriology Department in the late 1920s, Werkman faced a powerful professional dilemma: establishing a research identity that distinguished him from his colleagues with flourishing national and international reputations. His solution was to radically alter his research program from traditional bacteriology to a biochemistry program, which reflected the influence of the Dutch microbiologist/biochemist, Albert Jan Kluyver (1888–1956). Werkman was extremely successful in this career change. His laboratory made significant contributions to biochemistry, and Werkman achieved a notable degree of personal success. His career began in the shadow of his departmental bacteriological colleagues; within a decade he became the department's dominant research figure, as a biochemist. Werkman's personal success, however, had profound consequences for the disciplinary future of bacteriology at Iowa State.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen, Garland. 1978. Life Sciences in the Twentieth Century. Cambridge: Cambridge University Press.

    Google Scholar 

  • American Society for Microbiology. 1992. “List of Presidents.” Directory of Members, 1995. Souderton, PA: Data-Matic Systems Co.

    Google Scholar 

  • Amsterdamska, Olga. 1995. “Beneficent Microbes: The Delft School of Microbiology and its Industrial Connections.” In: Beijerinck and the Delft School of Microbiology, ed. Piet Bos and Bert Theunissen, pp. 193-213. Delft: Delft University Press.

    Google Scholar 

  • Andersen, Ariel A. 1945. “Letter to Max Levine, March 10, 1945.” Buchanan Papers, Box 3 (folder labeled: Levine vs. Werkman correspondence).

  • Anonymous. 1932a. “Holland Scientist to Speak at ISC.” Ames Daily Tribune (May 9, 1932): 1.

  • — 1932b. “Kluyver Here From Holland for Lectures.” Iowa State Daily (May 10, 1932): 1.

  • —1943. “Memo: Members of the Instructional and Research Staffs in Bacteriology, 1943.” American Society for Microbiology Archives. University of Maryland, Baltimore County, Record Group 13.II.T.

  • — 1945. “Three New Department Heads Named in Iowa State Division of Science.” Ames Daily Tribune (July 2, 1945).

  • Archival Index. Iowa State University, Iowa State University Archives, Ames, Iowa, Record Group 13/15.

  • Bechtel, William. 1986a. “Biochemistry: A Cross-Disciplinary Endeavor That Discovered a Distinctive Domain.” In: Integrating Scientific Disciplines, ed. W. Bechtel, pp. 77-100. Dordrecht: Martinus Nijhoff.

    Google Scholar 

  • — 1986b. “Building Interlevel Theories: The Discovery of the Embden-Meyerhoff Pathway and the Phosphate Cycle.” In: Foundations of Biology, ed. P. Weingartner and G. Dorn, pp. 65-97. Vienna: Hölder-Pichler-Tempsky Verlag.

    Google Scholar 

  • — 1988. “Fermentation Theory: Empirical Difficulties and Guiding Assumptions.” In: Scrutinizing Science: Empirical Studies of Scientific Change, ed. Arthur Donovan, Larry Laudan and Rachel Laudan, pp. 163-181. Baltimore: The Johns Hopkins University Press.

    Google Scholar 

  • Bennett, Joan and Phaff, Herman. 1993. “Early Biotechnology: The Delft Connection.” ASM News 59: 401-403.

    Google Scholar 

  • Bos, Piet and Theunissen, Bert (ed.). 1995. Beijerinck and the Delft School of Microbiology. Delft: Delft University Press.

    Google Scholar 

  • Brown, Russell W. 1974. “Chester Hamlin Werkman, 1893-1962.” Biographical Memoirs, National Academy of Sciences (U.S.) 44: 328-370.

    Google Scholar 

  • Buchanan, Robert Earle Papers. Iowa State University Archives, Ames, Iowa, Record Group 6/3/11. Referred to as Buchanan Papers.

  • —“On the Significance to Be Attributed to the Term Fermentation: II.” Kluyver Papers.

  • — 1918. “Life Phases in a Bacterial Culture.” Journal of Infectious Diseases 23: 109-125.

  • — 1931. “Annual Statement of Dean of the Graduate College to the Graduate Faculty, September 16, 1931.” Buchanan Papers, Box 6.

  • — 1932. “Annual Statement of Dean of Graduate College to the Graduate Faculty, Iowa State College, Sept. 24, 1932.” Buchanan Papers, Box 6.

  • — 1945a. “Letter to President Charles Friley, June 20, 1945.” Buchanan Papers, Box 3 (folder labeled: Levine vs. Werkman correspondence).

  • —1945b. “Letter to President Charles Friley, July 2, 1945.” Buchanan Papers, Box 3 (folder labeled: Levine vs. Werkman correspondence).

  • —1959. “Letter to Paul F. Clark, January 28, 1959.” Buchanan Papers, Box 2c.

  • Buchanan, Robert E. and Fulmer, Ellis I. 1928. Physiology and Biochemistry of Bacteria, Vol. I. Growth Phases; Composition, and Biophysical Chemistry of Bacteria and Their Environment; and Energetics. Baltimore: The Williams & Wilkins Company.

    Google Scholar 

  • — 1930a. Physiology and Biochemistry of Bacteria, Vol. II. Effects of Environment Upon Microörganisms. Baltimore: The Williams & Wilkins Company.

    Google Scholar 

  • — 1930b. Physiology and Biochemistry of Bacteria, Vol. III. Effects of Microörganisms Upon Environment. Fermentative and Other Changes Produced. Baltimore: TheWilliams & Wilkins Company.

    Google Scholar 

  • Budget: Iowa State College of Agriculture and Mechanical Arts, Iowa State University Archives (Ames, Iowa). Bound collection.

  • Eaborn, C. 1990. “Henry Gilman.” Biographical Memoirs of Fellows of the Royal Society 36: 153-172.

    Google Scholar 

  • Fleming, M. 1956. “Bibliography of Levine, M.” Papers of L. S. McClung, American Society for Microbiology Archives. University of Maryland, Baltimore County.

    Google Scholar 

  • Florkin, Marcel. 1975. A History of Biochemistry. Part III. History of the Identification of the Sources of Free Energy in Organisms. Vol. 31 of Comprehensive Biochemistry, ed. M. Florkin and E. H. Stotz. Amsterdam: Elsevier Scientific Publishing Company.

    Google Scholar 

  • Friley, Charles. 1945. “Letter to R. E. Buchanan, June 29, 1945.” Buchanan Papers, Box 3 (folder labeled: Levine vs. Werkman correspondence).

  • Fruton, Joseph S. 1990. Contrasts in Scientific Style. Philadelphia: American Philosophical Society.

    Google Scholar 

  • — 1992. A Skeptical Biochemist. Cambridge, MA: Harvard University Press.

    Google Scholar 

  • Gossel, Patricia P. 1992. “A Need for Standard Methods: The Case of American Bacteriology.” In: The Right Tools for the Job in Twentieth Century Life Sciences: Materials, Techniques, Instruments, Models and Work Organization, ed. Adele E. Clarke and Joan H. Fujimura, pp. 287-311, Princeton: Princeton University Press.

    Google Scholar 

  • Gunsalus, Irwin C. 1976. “Lessons in Learning.” In: Reflections on Biochemistry in Honor of Severo Ochoa, ed. Arthur Kornberg, Bernard L. Horecker, L. Cornudella and J. Oro, pp. 125-135. New York: Pergamon Press.

    Google Scholar 

  • Haldane, John Burdon Sanderson. 1938. “The Biochemistry of the Individual.” In: Perspectives in Biochemistry, ed. Joseph Needham and David E. Green. Cambridge: Cambridge University Press.

    Google Scholar 

  • Harrow, Benjamin. 1943. Textbook of Biochemistry, 3rd Edition. Philadelphia: W. B. Saunders.

    Google Scholar 

  • Hartman, Paul A. 1995. Letter to Author. April 24.

  • Holmes, Frederic L. 1981. “The Fine Structure of Scientific Creativity.” History of Science 19: 60-70.

    Google Scholar 

  • — 1986. “Intermediary Metabolism in the Early Twentieth Century.” In: Integrating Scientific Disciplines, ed. W. Bechtel. Dordrecht: Martinus Nijhoff.

    Google Scholar 

  • — 1991. Hans Krebs: The Formation of a Scientific Life, 1900-1933. New York: Oxford University Press.

  • — 1992. Between Biology and Medicine: The Formation of Intermediary Metabolism. Berkeley: Office for History of Science and Technology, University of California.

  • — 1993. Hans Krebs: The Architect of Intermediary Metabolism, 1933-1937. New York: Oxford University Press.

  • Hull, David L. 1988. Science as a Process. Chicago: University of Chicago Press.

    Google Scholar 

  • Iowa State College of Agricultural and Mechanical Arts Official Publication (Iowa State College). 1932. Catalogue Announcements for 1932-1933. Vol. XXX, No. 39. Ames, Iowa: Iowa State College of Agricultural and Mechanical Arts.

    Google Scholar 

  • Kamp, A. F., La Rivière, J. W. M. and Verhoeven, W. 1959. “Kluyver as Professor: Chronicles of the Laboratory.” In: Albert Jan Kluyver: His Life and Work, ed. A. F. Kamp, J. W. M. La Rivière and W. Verhoeven, pp. 14-48. New York: Interscience Publishers.

    Google Scholar 

  • Kay, Lily E. 1989. Molecules, Cells, and Life: An Annotated Bibliography of Manuscript Sources on Physiology, Biochemistry, and Biophysics, 1900-1960, in the Library of the American Philosophical Society. Philadelphia: American Philosophical Society Library.

    Google Scholar 

  • Kitcher, Philip. 1993. The Advancement of Science: Science Without Legend, Objectivity Without Illusions. New York: Oxford University Press.

    Google Scholar 

  • Kluyver, Albert Jan. Papers. Technical University of Delft, Delft, Holland. File drawer labeled: “Ames, etc.” Referred to as Kluyver Papers.

  • —“Lectures Ames.” Hand written, in English. List of dates and topics for the Ames lecture series. Kluyver Papers.

  • — “Microbial Metabolism.” Typed manuscript that served as lecture notes for the Ames lecture series. Kluyver Papers.

  • — “On the Significance to Be Attributed to the Term 'Fermentation'.” Kluyver Papers (Referred to as “Significance.”).

  • —“Student's Pocket Note Book.” Kluyver Papers.

  • — 1931. The Chemical Activities of Micro-Organisms. London: University of London Press.

  • — 1932a. “Microbial Metabolism and Its Bearing on the Cancer Problem.” Science 76: 527-532.

  • — 1932b. “Letter to C. H. Werkman, Chicago, Ill., August 9, 1932.” Privately held by Mr. Robert Werkman. I thank Mr. Werkman for sharing this letter with me and for permission to use it.

  • Kluyver, Albert J. and Donker, H. J. L. 1926. “Die Einheit in der Biochemie.” Chem. Zelle Gewebe 13: 134-190.

    Google Scholar 

  • Kluyver, Albert J. and Van Niel, Cornelius B. 1956. The Microbe's Contribution to Biology. Cambridge, MA: Harvard University Press.

    Google Scholar 

  • Kohler, Robert E. 1973. “The Enzyme Theory and the Origins of Biochemistry.” Isis 64: 181-196.

    Article  Google Scholar 

  • — 1982. From Medical Chemistry to Biochemistry: The Making of a Biomedical Discipline. Cambridge: Cambridge University Press.

    Google Scholar 

  • — 1985. “Innovation in Normal Science: Bacterial Physiology.” Isis 76: 162-181.

    Article  Google Scholar 

  • Korman, Ephraim F. 1974. “The Discovery of Fructose-1,6-Diphosphate (the Harden-Young Ester) in the Molecularization of Fermentation and of Bioenergetics.” Molecular and Cellular Biochemistry 5: 65-68.

    Article  Google Scholar 

  • Kornberg, Arthur. 1989. For the Love of Enzymes: The Odyssey of a Biochemist. Cambridge, Mass.: Harvard University Press.

    Google Scholar 

  • Laszlo, Pierre. 1986. Molecular Correlates of Biological Concepts. Vol. 34A of Comprehensive Biochemistry, ed. A. Neuberger and L. L. van Deenen. Amsterdam: Elsevier Scientific Publishing Company.

    Google Scholar 

  • Levine, Max. 1918a. “Differentiation of B. coli and B. aerogenes on a Simplified Methylene Blue Agar.” Journal of Infectious Diseases 23: 43-47.

    Google Scholar 

  • — 1918b. “Presumptive Tests for B. coli.” Journal of the American Water Works Association 5: 168-171.

    Google Scholar 

  • Levine, Max and Buchanan, J. H. 1928. “Some Factors Influencing the Germicidal Efficiency of Alkalies.” American Journal of Public Health 28: 1361-1368.

    Article  Google Scholar 

  • Levine, Max, Toulouse, J. and Buchanan, J. H. 1928. “Effect of Addition of Salts on the Germicidal Efficiency of Sodium Hydroxide.” Industrial and Engineering Chemistry 20: 179-184.

    Article  Google Scholar 

  • Lewis, Sinclair. 1980. Arrowsmith. New York: Penguin Books.

    Google Scholar 

  • Monod, Jacques and Jacob, François. 1961. “General Conclusions: Teleonomic Mechanisms in Cellular Metabolism, Growth and Differentiation.” Cold Spring Harbor Symposia On Quantitative Biology (Cellular Regulatory Mechanisms) 26: 389-401.

    Google Scholar 

  • Morgan, Neil. 1985. “Pure Science and AppliedMedicine: The Relationship Between Bacteriology and Biochemistry in England After 1880.” Bulletin of the Society for the Social History of Medicine 37: 46-49.

    Google Scholar 

  • — 1990. “From Physiology to Biochemistry.” In: Companion to the History of Modern Science, ed. Robert C. Olby, Geoffrey Cantor, John Christie and Jonathon Hodge, pp. 494-501. London: Routledge.

    Google Scholar 

  • Neuberg, Carl. 1932. “Letter to C. H. Werkman, August 8, 1932.” Werkman Papers, Box 2. I thank Alan Rocke for assistance with the translation.

  • Packer, Raymond A. 1958. “Bacteriology at Iowa State College.” Unpublished manuscript. American Society for Microbiology Archives, University of Maryland, Baltimore County.

    Google Scholar 

  • — 1983. “Early History of the Teaching of Veterinary Bacteriology at Iowa State University.” Unpublished manuscript. American Society for Microbiology Archives, University of Maryland, Baltimore County.

    Google Scholar 

  • Rosenberg, Charles E. 1976. “Martin Arrowsmith: The Scientist as Hero.” In: No Other Gods: On Science and American Social Thought, pp. 123-131. Baltimore: Johns Hopkins University Press.

    Google Scholar 

  • Rossiter, Margaret. 1986. “Graduate work in the Agricultural Sciences, 1900-1970.” Agricultural History 60: 37-57.

    Google Scholar 

  • Sedgwick, W. T. 1913. “Letter to Max Levine, July 25, 1913” (Privately held by Mrs. Thelma Levine Worthen). I thank Mrs. Worthen for sharing this letter with me and for permission to use it.

  • Sinsheimer, Robert. 1994. The Strands of a Life: the Science of DNA and the Art of Education. Berkeley: University of California Press.

    Google Scholar 

  • —1998. Electronic Correspondence to the Author, April 19.

  • Singleton, Rivers Jr. 1997a. “Heterotrophic CO2-Fixation, Mentors, and Students: The Wood-Werkman Reaction.” The Journal of the History of Biology 30: 91-120.

    Article  Google Scholar 

  • — 1997b. “Harland Goff Wood: An American Biochemist.” In: Comprehensive Biochemistry: History of Biochemistry, Volume 40, ed. G. Semenza and R. Jaenicke. Amsterdam: Elsevier.

    Google Scholar 

  • — 2000. “Robert Earle Buchanan: An Unappreciated Scientist.” Yale Journal of Biology and Medicine: in press.

  • Stigler, George J. 1956. Trends in Employment in the Service Industries: A Study by the National Bureau of Economic Research. Princeton: Princeton University Press.

    Google Scholar 

  • Teich, Mikuláš. 1970. “The Historical Foundations of Modern Biochemistry.” In: The Chemistry of Life, ed. Joseph Needham. Cambridge: Cambridge University Press.

    Google Scholar 

  • Theunissen, Bert. 1996. “The Beginnings of the 'Delft Tradition' Revisited: Martinus W. Beijerinck and the Genetics of Microorganisms.” The Journal of the History of Biology 29: 197-228.

    Article  Google Scholar 

  • Van Niel, Cornelius B. 1959. “Kluyver's Contributions to Microbiology and Biochemistry.” In: Albert Jan Kluyver: His Life and Work, ed. A. F. Kamp, J. W. M. La Rivière and W. Verhoeven, pp. 68-155. New York: Interscience Publishers.

    Google Scholar 

  • Werkman, Chester Hamlin. Papers. Iowa State University Archives, Ames, Iowa, Record Group 13/15/12. Referred to asWerkman Papers.

  • —“Critique of Recent Theories of Fermentation.” Werkman Papers, Box 1.

  • — 1939. “Bacterial Dissimilation of Carbohydrates.” Bacteriological Reviews 3: 187-227.

  • — 1946. “Letter to F. E. Wright, May 6, 1946.” National Academy of Science Archives, Washington, DC. Record Group. ADM: NAS: Members, Werkman, C. H.

  • Werkman, Chester H. and Wood, Harland G. 1942. “On the Metabolism of Bacteria.” Botanical Review 8: 1-68.

    Article  Google Scholar 

  • Worthen, Thelma Levine. 1995. Letter to the Author, November 17.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singleton, R. From Bacteriology to Biochemistry: Albert Jan Kluyver and Chester Werkman at Iowa State. Journal of the History of Biology 33, 141–180 (2000). https://doi.org/10.1023/A:1004775817881

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1004775817881

Navigation