Abstract
The Al accumulation mechanisms in an Al accumulator plant, Melastoma malabathricum L. (Melastoma), was investigated. Al was located in the upper epidermal cells and also distributed in mesophyll cells in leaf sections. In root sections, Al was found in all the root tissues, particularly in the epidermis and endodermis. Al concentrations in young leaves, mature leaves, old leaves, and roots were 8.0, 9.2, 14.4, and 10.1 mg g1, respectively. Approximately 45% of total Al in oldest leaves, and approximately 60% of total Al in leaves of other positions and roots were extracted in Tris-HCl buffer (pH 7.0). Since Al in the residual parts was mostly dissolved in hot 0.5 M H2SO4 containing 2% cetyl trimethylammonium bromide, residual Al seemed to consist mainly of monomeric Al and Al bound to pectic substances and hemicellulose. Al in the Tris-HCl extract consisted of non-monomeric Al (complexed form). Oxalate concentration in the Tris-HCl extract in leaves was significantly higher in the +Al treatment than in the –Al treatment and there was a positive correlation between the Al concentration and oxalate concentration. 27Al NMR spectrum of fresh leaves indicated the presence in the order of monomeric Al, Al-oxalate, Al-(oxalate)2, and Al-(oxalate)3 in intact leaves.
Similar content being viewed by others
References
Aniol A 1984 Induction of aluminum tolerance in wheat seedlings by low doses of aluminum in the nutrient solution. Plant Physiol. 75, 551–555.
Chenery E M 1948 Aluminium in the plant world. Part I. Kew Bull. 2, 173–183.
Cuenca G, Herrera R and Medina E 1990 Aluminium tolerance in trees of a tropical cloud forest. Plant Soil 125, 169–175.
Cuenca G, Herrera R and Mérida T 1991 Distribution of aluminium in accumulator plants by X-ray microanalysis in Richeria grandis Vahl leaves from a cloud forest in Venezuela. Plant Cell Environ. 14, 437–441.
Delhaize E and Ryan P R 1995 Aluminum toxicity and tolerance in plants. Plant Physiol. 107, 315–321.
Delhaize E, Craig S, Beaton C D, Bennet R J, Jagadish V C and Randall P J 1993 Aluminium tolerance in wheat (Triticum aestivum L.). I. Uptake and distribution of aluminium in root apices. Plant Physiol. 103, 685–693.
Foy C D, Chaney R L and White M C 1978 The physiology of metal toxicity in plants. Ann. Rev. Plant Physiol. 29, 511–566.
Foy C D, Lee E H, Coradetti C A and Taylor G J 1990 Organic acids related to differential aluminium tolerance in wheat (Triticum aestivum) cultivars. In Plant Nutrition — Physiology and Applications. Ed. M L van Beusichem. pp 381–389. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Geoghegan I E and Sprent J I 1996 Aluminum and nutrient concentrations in species native to central Brazil. Commun. Soil Sci. Plant Anal. 27, 2925–2934.
Grill E, Winnacker E-L and Zenk M H 1985 Phytochelatins: The principal heavy-metal complexing peptides of higher plants. Science 230, 674–676.
Haridasan M 1982 Aluminium accumulation by some cerrado native species of central Brazil. Plant Soil 65, 265–273.
Haridasan M 1985 Accumulation of nutrients by eucalyptus seedlings from acidic and calcareous soils of the cerrado region of central Brazil. Plant Soil 86, 35–45.
Haridasan M, Paviani T I and Schiavini I 1986 Localization of aluminium in the leaves of some aluminium-accumulating species. Plant Soil 94, 435–437.
Haridasan M 1988 Performance of Miconia albicans (SW.) Triana, an aluminum accumulating species, in acidic and calcareous soils. Commun. Soil Sci. Plant Anal. 19, 1091–1103.
Ishikawa S, Wagatsuma T and Ikarashi T 1996 Comparative toxicity of Al3+, Yb3+, and La3+ to root-tip cells differing in tolerance to high Al3+ in terms of ionic potentials of dehydrated trivalent cations. Soil Sci. Plant Nutr. 42, 613–625.
Kerven G L, Edwards D G, Asher C J, Hallman P S and Kokot S 1989 Aluminium determination in soil solution. II. Short term colorimetric procedures for the measurement of inorganic monomeric aluminium in the presence of organic acid ligands. Aust. J. Soil Res. 27, 91–102.
Kerven G L, Larsen P L, Bell L C and Edwards D G 1995 Quantitative 27Al NMR spectroscopic studies of Al(III) complexes with organic acid ligand and their comparison with GEOCHEM predicted values. Plant Soil 171, 35–39.
Kochian L V 1995 Cellular mechanisms of aluminum toxicity and resistance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46, 237–260.
Ma J F, Hiradate S, Nomoto K, Iwashita T and Matsumoto H 1997 Internal detoxification mechanism of Al in Hydrangea. Plant Physiol. 113, 1033–1039.
Marschner H 1991 Mechanisms of adaptation of plants to acid soils. Plant Soil 134, 1–20.
Matsumoto H, Hirasawa E, Morimura S and Takahashi E 1976 Localization of aluminium in tea leaves. Plant Cell Physiol. 17, 627–631.
Mizuno N and Minami M 1980 The use of H2SO4-H2O2 for the destruction of plants matter as a preliminary to determination of N, K, Mg, Ca, Fe, Mn. Jpn. J. Soil Sci. Plant Nutr. 51, 418–420. (In Japanese).
Nagata T, Hayatsu M and Kosuge N 1992 Identification of aluminium forms in tea leaves by 27Al NMR. Phytochem. 31, 1215–1218.
Osaki M, Sittibush C and Nuyim T 1995 Nutritional characteristics of wild plants grown in peat and acid sulfate soils distributed in Thailand and Malaysia. In A Tropical Swamp Forest Ecosystem and Its Greenhouse Gas Emission. Eds. P Vijarsorn, K Suzuki, K Kyuma, E Wada, T Nagano and Y Takai. pp 63–76. Nodai Research Institute Tokyo University of Agriculture, Tokyo.
Osaki M, Watanabe T and Tadano T 1997 Beneficial effect of aluminum on growth of plants adapted to low pH soils. Soil Sci. Plant Nutr. 43, 827–837.
Osaki M, Shinano T and Tadano T 1991 Redistribution of carbon and nitrogen compounds from the shoot to the harvesting organs during maturation in field crops. Soil Sci. Plant Nutr. 37, 117–128.
Pellet D M, Grunes D L and Kochian L V 1995 Organic acid exudation as an aluminum-tolerance mechanism in maize (Zea mays L.). Planta 196, 788–795.
Putterill J J and Gardner R C 1988 Proteins with the potential to protect plants from Al3+ toxicity. Biochim. Biophys. Acta 964, 137–145.
Van Soest P J 1963a Use of detergents in the analysis of fibrous feeds. I. Preparation of fiber residues of low nitrogen content. J. Assoc. Off. Agric. Chem. 46, 825–829.
Van Soest P J 1963b Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fiber and lignin. J. Assoc. Off. Agric. Chem. 46, 829–835.
Takeda K, Kubota R and Yagioka C 1985a Copigments in the blueing of sepal color of Hydrangea macrophylla. Phytochemistry 24, 1207–1209.
Takeda K, Kariuda M and Itoi H 1985b Blueing of sepal color of Hydrangea macrophylla. Phytochemistry 24, 2251–2254.
Tice K R, Parker D R and DeMason D A 1992 Operationally defined apoplastic and symplastic aluminum fractions in root tips of aluminum-intoxicated wheat. Plant Physiol. 100, 309–318.
Trevelyan A and Harrison J S 1952 Studies on yeast metabolism 1. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50, 298–303.
Vance G F, Stevenson F J and Sikora F J 1996 Environmental chemistry of aluminum-organic complexes. In The Environmental Chemistry of Aluminum. Ed. G Sposito. pp 169–220. CRC Press, Inc., Boca Raton.
Watanabe T, Osaki M and Tadano T 1997 Aluminum-induced growth stimulation in relation to calcium, magnesium, and silicate nutrition in Melastoma malabathricum L. Soil Sci. Plant Nutr. 43, 827–837.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watanabe, T., Osaki, M., Yoshihara, T. et al. Distribution and chemical speciation of aluminum in the Al accumulator plant, Melastoma malabathricum L.. Plant and Soil 201, 165–173 (1998). https://doi.org/10.1023/A:1004341415878
Issue Date:
DOI: https://doi.org/10.1023/A:1004341415878