Article

Antonie van Leeuwenhoek

, Volume 73, Issue 1, pp 127-141

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

  • Gerard MuyzerAffiliated withMax-Planck-Institute for Marine Microbiology
  • , Kornelia SmallaAffiliated withBiologische Bundesanstalt für Land- und Forstwirtschaft

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Abstract

Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.

DGGE genetic fingerprinting microbial ecology molecular microbial ecology PCR rRNA TGGE