Antonie van Leeuwenhoek

, Volume 73, Issue 1, pp 127–141

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

Authors

  • Gerard Muyzer
    • Max-Planck-Institute for Marine Microbiology
  • Kornelia Smalla
    • Biologische Bundesanstalt für Land- und Forstwirtschaft
Article

DOI: 10.1023/A:1000669317571

Cite this article as:
Muyzer, G. & Smalla, K. Antonie Van Leeuwenhoek (1998) 73: 127. doi:10.1023/A:1000669317571

Abstract

Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.

DGGEgenetic fingerprintingmicrobial ecologymolecular microbial ecologyPCRrRNATGGE

Copyright information

© Kluwer Academic Publishers 1998