Abstract
To obtain recombinant restriction endonuclease (R) and methylase (M) of the EcoRII restriction–modification system, bacterial strains overproducing their functional hexahistidine derivatives were constructed. Active full-length R · EcoRII was produced only in cells that also expressed M · EcoRII from a multicopy plasmid. Recombinant R · EcoRII bound with hybrid DNA · RNA duplexes.
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Babkina, O.V., Evstafieva, A.G., Chichkova, N.V. et al. Recombinant Components of the EcoRII Restriction–Modification System: Restriction Endonuclease Can Interact with DNA · RNA Duplexes. Molecular Biology 34, 913–920 (2000). https://doi.org/10.1023/A:1026688028427
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DOI: https://doi.org/10.1023/A:1026688028427