Pollen viability as a bio-indicator of air quality
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Many air pollutants cause plant deterioration. In this study pollen viability was used as bio-indicator of air quality. The study was carried out in the city of Perugia where road traffic is the most important cause of air pollution.
Three areas, with different intensity of road traffic (very high, medium and absent) but all characterized by the presence of the same plant species, were selected.
Eight species were studied: Hedera helix L., Convolvulus sepium L., Cynodon dactylon (L.) Pers., Quercus ilex L., Dactylis glomerata L., Parietaria diffusa M. et K., Daucus carota L. and Tilia cordata Miller. The pollen of these species was treated with TTC (2, 3, 5 Tryphenil-Tetrazolium-Chloride) staining solution and viability was then estimated by light microscopy. The results showed that the pollen viability was inversely proportioned with pollution. The highest difference in pollen viability between the areas was registered in Tilia cordata. Quercus ilex instead showed that there was no difference in pollen viability between the three different areas. Parietaria diffusa showed a particular behaviour; the highest pollen viability percentage was in polluted areas.
The statistical analysis (ANOVA) showed that the main source of variability of the pollen viability depends on the plant but also the site and the interaction between plant and site were very important with a high significant level (p < 0.0001).
- Alexander M.P.: 1969, Differential staining of aborted and nonaborted pollen. Stain Technology 44(3), 117–122.
- Bellani L.M., Paoletti E. and Cenni E.: 1988, Air pollution effects on pollen germination of Forest species. In: Cresti (ed), Sexual Reproduction in Higher Plants, pp. 265–270.
- Bricchi E., Mincigrucci G., Frenguelli G., Iannotti O., Cingolani L., Di Brizio M. and Morosi A.: 1998, Pollen: Bio-indicator of Air Quality. Abs. of 6th International Congress of Aerobiology, Perugia (Italy).
- Comtois P. and Schemenauer R.S.: 1991, Tree pollen viability in areas subject to high pollutant deposition. Aerobiologia 7, 144–151. CrossRef
- Comtois P.: 1994, Airborne pollen dispersal and survival on Mount Sutton (Canada). Aerobiologia 10, 31–37. CrossRef
- Franchi G.G., Pacini E. and Rottoli P.: 1984, Pollen grain viability in Parietaria judaica L. during the long blooming period and correlation with meteorological conditions and allergic diseases. Giorn. Bot. Ital. 118, 163–178.
- Melati M.R., Alaimo M.G., Bruno A., Palmeri E. and Todaro C.: 1997, Riscontro di piombo in piante erbacee con piccoli pollini (Parietaria). Not. Allergol. 16, 91–96.
- Sarvella P.: 1964, Vital-stain testing of pollen viability in cotton. Journal of Heredity 55, 154–158.
- Pollen viability as a bio-indicator of air quality
Volume 16, Issue 3-4 , pp 361-365
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- Kluwer Academic Publishers
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- air quality
- Author Affiliations
- 1. Department of Plant Biology, University of Perugia, Borgo XX giugno 74, I-06100, Perugia, Italy
- 2. Department of Plant Biology, University of Perugia, Italy
- 3. Department of Plant Biology, University of Perugia, Italy (Author for correspondence, e-mail: