Abstract
It is likely that in the near future sequence information from sequencing programmes and EST libraries will generate an abundance of genic microsatellite markers. This study is focused on the assessment of their likely impact and performance vis-à-vis their genomic counterparts. Microsatellites from two sources were used to assess the genetic diversity in 56 old and new varieties of bread wheat on the UK Recommended List. A set of 12 microsatellite markers generated from genomic libraries and 20 expressed sequence tag (EST)-derived microsatellites were used in the study, and the performance of both marker sets assessed. The EST-derived or genic microsatellites delivered fingerprints of superior quality, amplifying clear products with few stutter bands. Diversity levels as revealed bygenic microsatellites are similar to the few published results. The PIC values for the genic markers were generally lower than those calculated for the genomic microsatellites, though advantages of both marker classes for variety identification applications are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson, J.A., G.A. Churchill, J.E. Autrique, S.D. Tanksley & M.E. Sorrells, 1993. Optimising parental selection for genetic linkage maps. Genome 36: 181-186.
Bryan, G.J., A.J. Collins, P. Stephenson, A. Orry, J.B. Smith & M.D. Gale, 1997. Isolation and characterisation of microsatellites from hexaploid bread wheat. Theor Appl Genet 94: 557-563.
Cho, Y.G., T. Ishii, S. Temnykh, X. Chen, L. Lipovich, S.R. Mc-Couch, W.D. Park, N. Ayres & S. Cartinhour, 2000. Diversity of microsatellites derived from genomic libraries and GenBank sequenes in rice (Oryza sativa L.). Theor Appl Genet 100: 713-722.
Donini, P., P. Stephenson, G.J. Bryan & R.M.D. Koebner, 1998. The potential of microsatellites for high throughput genetic diversity assessment in wheat and barley. Gen Res Crop Evol 45: 415-421.
Donini, P., R.J. Cooke & J.C. Reeves, 2000a. Molecular markers in variety and seed testing. In: J.P. Gustafson (Ed.), Plant Genetic Engineering: Towards the Third Millennium, pp. 27-34. AD Arencibia, Elsevier Science.
Donini, P., J.R. Law, R.M.D. Koebner, J.C. Reeves & R.J. Cooke, 2000b. Temporal trends in the diversity of UK wheat. Theor Appl Genet 100: 912-917.
Eujayl, I., M.E. Sorrells, M. Baum, P. Wolters & W. Powell, 2001. Assessment of genotype variation among cultivated durum wheat based on EST-SSRs and genomic SSRs. Euphytica 119: 39-43.
Eujayl, I., M.E. Sorrells, M. Baum, P. Wolters & W. Powell, 2002. Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor Appl Genet 104: 399-407.
Flavell, R.B., M.D. Bennet, J.B. Smith & D.B. Smith, 1974. Genome size and the proportion of repeated nucleotide sequence DNA in plant. Biochem Genet 12: 257-269.
Gale, M.D., S. Chao & P.J. Sharp, 1990. RFLP mapping in wheat: progress and problems. In: Proc 19th Stadler Genet Symp, pp. 353-363. Plenum Press, New York.
Karagyosov, L., I. Kalcheve & V. Chapman, 1993. Construction of libraries enriched for sequence specific repeats. Nucl Acids Res 21: 3911-3912.
Koebner, R.M.D., W. Powell & P. Donini, 2001. The contribution of current and forthcoming DNA molecular marker technologies to wheat and barley genetics and breeding. In: J. Janick (Ed.), Plant Breeding Reviews, pp. 181-220, Volume 21. John Wiley & Sons, Inc.
Law, J.R., R.M.D. Koebner, P. Donini, J.C. Reeves & R.J. Cooke, 1998. DNA profiling and plant variety registration. 3: The statistical assessment of distinctness in wheat using amplified fragment length polymorphisms. Euphytica 102: 335-342.
Morgante, M., M. Hanafey & W. Powell, 2002. Microsatellites are preferentially associated with norepetitive DNA in plant genomes. Nature Genet 30(2): 194-200.
Scott, K.D., P. Eggler, G. Seaton, M. Rossetto, E.M. Ablett, L.S. Lee & R.J. Henry, 2000. Analysis of SSRs derived from grape ESTs. Theor Appl Genet 100: 723-726.
Van der Peer, Y. & R. DeWachter, 1994. TREECON for windows: a software package for the construction and drawing of evolutionary trees for the microsoft windows environment. Comput Applic Biosci 10: 569-570.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Leigh, F., Lea, V., Law, J. et al. Assessment of EST- and genomic microsatellite markers for variety discrimination and genetic diversity studies in wheat. Euphytica 133, 359–366 (2003). https://doi.org/10.1023/A:1025778227751
Issue Date:
DOI: https://doi.org/10.1023/A:1025778227751