In vitro plantlet regeneration from cotyledons of the tree-legume Leucaena leucocephala
Cite this article as: Saafi, H. & Borthakur, D. Plant Growth Regulation (2002) 38: 279. doi:10.1023/A:1021591212710 Abstract
Two plant regeneration methods applicable to
Leucaenaleucocephala were developed. In the first method, involvingorganogenesis via callus formation, cotyledon, hypocotyl and root segments wereinitiated on MS medium containing different concentrations ofN 6-benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), andnaphthaleneacetic acid (NAA). Both compact (type I) and friable (type II) calliwere obtained from the cotyledon and hypocotyl explants treated with differentconcentrations of the growth regulators. Shoots were generated only from thefriable calli formed from the cotyledon explants. The calli formed from thehypocotyl explants did not generate shoots and the root explants died withoutforming callus. Cotyledon explants from 3–4 day old seedlings showedmaximum callus induction compared to those from older seedlings. In a secondmethod involving direct organogenesis, excised cotyledons were cultured on 1/2MS medium containing 10–35 mg l −1N 6-benzyladenine (BA) for 7–14 days. Transfer of thecotyledonsto regeneration medium containing low BA resulted in callus formation andsubsequent shoot regeneration from the base of the excised cotyledon explants,with up to 100% frequency. Regenerated shoots rooted best on a basal mediumcontaining no growth regulators. Callus Leucaena leucocephala Organogenesis Tissue culture References
Armstrong C.L. and Green C.E. 1985. Establishment and maintenance of friable, embryogenic maize callus and involvement of L-proline. Planta 164: 207–214.
Brar M.S., Al-khayri J.M., Morelock T.E. and Anderson E.J. 1999. Genotypic response of Cowpea
regeneration from cotyledon explants. In Vitro Cell. Dev. Biol.-Plant 35: 8–12.
Buiteveld J., Fransz P.F. and Creemers-Molenaar J. 1994. Induction and characterization of embryogenic callus types for the initiation of suspension cultures of leek (
L.). Plant Sci. 100: 195–202.
Burnett L., Arnoldo M., Yarrow S. and Huang B. 1994. Enhancement of shoot regeneration from cotyledon explants of
through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell, Tiss. Organ Cult. 37: 253–256.
Chang C.C., Moll C.B., Evenson K.B. and Guiltinan M.J. 1996.
plantlet regeneration from cotyledon, hypocotyl and root explants of hybrid seed geranium. Plant Cell, Tiss. Organ Cult. 45: 61–66.
Das P.K., hakravarti V. and Maity S. 1993. Plantlet formation in tissue culture from cotyledon of
A. Cunn ex Benth. Ind. J. Forest. 16: 189–192.
Gendy C., Sene M., Le B.V., Vidal J. and Van K.T.T. 1996. Somatic embryogenesis and plant regeneration in
Sorghum bicolor (L.). Moench. Plant Cell Rep. 15: 900–904.
Gill R. and Ozias-akins P. 1999. Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (
L.) plants. In vitro Cell. Dev. Biol.-Plant 35: 445–450.
Glovak L. and Greatbatch W. 1981. Successful tissue culture of
. Leucaena Res. Rep. 3: 81–82.
Goyal Y., Bingham R.L. and Felker P. 1995. Progragation of tropical tree, Leucaena leucocephala K67, by
bud culture. Cell Tissue Organ Cult. 2: 49–53.
Jin R.-G., Liu Y.-B., Tabashnik B.E. and Borthakur D. 2000. Development of transgenic cabbage (
) for insect resistance by
-mediated transformation. In Vitro Cell. Dev. Biol.-Plant 36: 231–237.
Jones R.J. 1979. The value of
as a feed for ruminants in the tropics. World Animal Rev. 31: 13–23.
Kuo Y.J. and Smith M.A.L. 1993. Plant regeneration from St. Augustine grass immature embryo-derived callus. Crop Sci. 33: 1394–1396.
Lin H.-S., van der Toorn C., Raemakers K.J.J.M., Visser F., De Jeu M.J. and Jacobsen E. 2000. Development of a plant regeneration system based on friable embryogenic callus in the ornamental
. Plant Cell Rep. 19: 529–534.
Mao A.A., Wetten A., Fay M.F. and Caligari P.D.S. 2000. In vitro propagation of
(Lours.) Pers., a multipurpose tree. Plant Cell Rep. 19: 263–267.
Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473–479.
Nagl W., Ignacimuthu S. and Becker J. 1997. Genetic engineering and regeneration of
: State of the art and new attempts. J. Plant Physiol. 150: 625–644.
Peaseley E.L. and Collins G.B. 1980. Development of an
culture system for
. Leucaena Newslett. 1: 54.
Sharma S.K. and Ramamurthy V. 2000. Micropropagation of 4-year-old elite
trees. Plant Cell Rep. 19: 511–518.
Shelton H.M. and Brewbaker J.L. 1994.
-the most widely used forage three legume. In: Gutteridge R.C. and Shelton H.M. (eds), Forest Tree Legumes in Tropical Agriculture. CAB International, Wallingford, UK, pp. 15–30.
Soedarjo M. and Borthakur D. 1996. Mimosine produced by the tree-legume
provides growth advantages to some
strains that utilizes it as a source of carbon and nitrogen. Plant Soil 186: 87–92.
Sunyal M., Gupta S.D., Jana M.K. and Kundu S.C. 1998. Shoot organogenesis and plant regeneration from leaf callus culture of Tuberose (
L.). Plant Tiss. Cult. Biotechnol. 4: 81–86.
Thomas T.D., Bhatnagar A.K. and Bhojiwani S.S. 2000. Production of triploid plants of mulberry (
Morus alba L.) by endosperm culture. Plant Cell Rep. 19: 395–399.
Tuskan G.A., Sargent W.A., Rensema T. and Walla J.A. 1990. Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of
(Dougl. ex Law) cotyledon explants. Plant Cell, Tiss. Organ Cult. 20: 47–52.
Google Scholar Copyright information
© Kluwer Academic Publishers 2002