Biotechnology Letters

, Volume 24, Issue 19, pp 1637–1641

Surface and physico-chemical properties of a glycolipid biosurfactant, mannosylerythritol lipid, from Candidaantarctica

  • Hee-Sik Kim
  • Jong-Woon Jeon
  • Seong-Bin Kim
  • Hee-Mock Oh
  • Tae-Jong Kwon
  • Byung-Dae Yoon
Article

DOI: 10.1023/A:1020309816545

Cite this article as:
Kim, H., Jeon, J., Kim, S. et al. Biotechnology Letters (2002) 24: 1637. doi:10.1023/A:1020309816545

Abstract

Candida sp. SY16 produces a glycolipid-type biosurfactant, mannosylerythritol lipid (MEL-SY16) which, at low concentrations, emulsifies hydrocarbons, vegetable oil, and crude oil. The molecular weight of MEL-SY16 was 634 Da and its hydrophilic lipophilic balance (HLB) was 8.8. It decreased the surface tension to 30 dyne cm−1 at a critical micelle concentration (CMC) of 15.8 μM, and the minimum interfacial tension against kerosene (a mixture of petroleum hydrocarbons) was 0.1 dyne cm−1. MEL-SY16 was stable from pH 4 to pH 10, up to 90 °C for 1 h, and against NaCl up to 100 mM. It was readily degraded by microorganisms in activated sludge within a short time, and exhibited very low toxicity to mouse fibroblast cells. These properties confirm that MEL-SY16 is suitable for a variety of environmental applications.

biosurfactantCandida antarcticamannosylerythritol lipidphysico-chemical propertiessurface activity

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Hee-Sik Kim
    • 1
  • Jong-Woon Jeon
    • 1
  • Seong-Bin Kim
    • 1
  • Hee-Mock Oh
    • 1
  • Tae-Jong Kwon
    • 2
  • Byung-Dae Yoon
    • 1
  1. 1.Laboratory of Environmental BiotechnologyKorea Research Institute of Bioscience and BiotechnologyYusong, TaejonKorea
  2. 2.Department of Microbial EngineeringKon-Kuk UniversitySeoulKorea