Digestive Diseases and Sciences

, Volume 43, Issue 2, pp 290–299

Specificity of Polymerase Chain Reaction Monoclonality for Diagnosis of Gastric Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma (Direct Comparison to Southern Blot Gene Rearrangement)

  • Allan P. Weston
  • Sushanta K. Banerjee
  • Rebecca T. Horvat
  • Rachel Cherian
  • Donald R. Campbell
  • Mikhail N. Zoubine
Article

DOI: 10.1023/A:1018842002926

Cite this article as:
Weston, A.P., Banerjee, S.K., Horvat, R.T. et al. Dig Dis Sci (1998) 43: 290. doi:10.1023/A:1018842002926

Abstract

The specificity of polymerase chain reactionmonoclonality in the diagnosis of gastric lymphoma wasprospectively evaluated. Gastric mucosal tissue fromnormal gastric mucosa (N = 13), benign gastric ulcers (N = 3), chronic Helicobacter pylori gastritis(N = 3), gastric mucosa-associated lymphoid tissue (N =16), and gastric lymphoma (N = 15) was obtained.Polymerase chain reaction amplification of theheavy-chain framework 2A gene was performed. Thesensitivity and specificity of heavy-chain clonality, inthe detection of gastric lymphoma, were 73.3% and 45.7%,respectively. Determination of monoclonality bypolymerase chain reaction methodology is not an acceptabletechnique for confirming the diagnosis of gastriclymphoma as it is too sensitive, detecting minutepopulations of clonal lymphocytes that occur in benign diseases as well as larger populations ofclonal lymphocytes associated with malignant gastriclymphoproliferative diseases. Southern blot generearrangement testing should be utilized to determineclonality in the evaluation of gastric lymphocyticinfiltrates.

HELICOBACTER PYLORIIMMUNOGLOBULIN GENE REARRANGEMENTSLYMPHOMAMUCOSA-ASSOCIATED LYMPHOID TISSUEPOLYMERASE CHAIN REACTION

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Allan P. Weston
  • Sushanta K. Banerjee
  • Rebecca T. Horvat
  • Rachel Cherian
  • Donald R. Campbell
  • Mikhail N. Zoubine

There are no affiliations available