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Direct somatic embryogenesis from mature embryos of sandalwood

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Abstract

Plants were regenerated from mature zygotic embryos of sandalwood (Santalum album L.) through direct somatic embryogenesis. Somatic embryos were formed directly without any intervening callus phase on zygotic embryos plated on Murashige and Skoog (MS) medium containing thidiazuron or benzylaminopurine. Individual somatic embryos were then isolated and transferred to MS medium without cytokinin on which they formed secondary embryos in repetitive cycles with or without the addition of indole acetic acid to the medium. Conversion of somatic embryos into plantlets was achieved by isolating somatic embryos with distinct cotyledons and reculturing them onto half-strength MS medium with GA3 (1.4 μM). Recovered plantlets were acclimatised and grown in the greenhouse. This is the first report on in vitro regeneration via direct somatic embryogenesis of sandalwood.

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References

  • Arrillaga I, Tobolski J & Merkle SA (1994) Advances in somatic embryogenesis and plant production of black locust (Robinia pseudoacacia L.). Plant Cell Rep. 13: 171–175

    Google Scholar 

  • Bapat VA & Rao PS (1979) Somatic embryogenesis and plantlet formation in tissue cultures of sandalwood (Santalum album L.). Ann. Bot. 44: 629–630

    Google Scholar 

  • Cuenca B, San-Jose MC, Martinez MT, Ballester A & Vieitez A (1999) Somatic embryogenesis from stem and leaf explants of Quercus robur L. Plant Cell Rep. 18: 538–543

    Google Scholar 

  • Dunstan DI, Tautorus TE & Thorpe TA (1995) Somatic embryogenesis in woody plants. In: Thorpe TA (ed) In Vitro Embryogenesis in Plants (pp 471–537). Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Ellis DD, Barczynska H & McCown BH (1991) A comparison of BA, zeatin and thidiazuron for adventitious bud formation from Picea glauca embryos and epicotyl explants. Plant Cell Tiss. Org. Cult. 27: 281–287

    Google Scholar 

  • Gharyal PK & Maheshwari SC (1981) In vitro differentiation of somatic embryoids in a leguminous tree - Albizzia lebbeck L. Naturewissenschaften 68: 379–380

    Google Scholar 

  • Huetteman CA & Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119

    Google Scholar 

  • Lakshmi Sita G, Raghava Ram NV & Vaidyanathan CS (1979) Differentiation of embryoids and plantlets from shoot callus of sandalwood. Plant Sci. Lett. 15: 265–270

    Google Scholar 

  • Lakshimi Sita G, Raghava Ram NV & Vaidyanathan CS (1980) Triploid plantlets from endosperm culture of sandalwood by experimental embryogenesis. Plant Sci. Lett. 20: 63–69

    Google Scholar 

  • Merkle SA (1995) Strategies for dealing with limitations of somatic embryogenesis in hardwood trees. Plant Tiss. Cult. Biotechnol. 1: 112–121

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Murthy BNS, Murch SJ & Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev. Biol. Plant 34: 267–275

    Google Scholar 

  • Murthy BNS & Saxena PK (1999) Somatic embryogenesis and plantregeneration of neem (Azadirchta indica A. Juss). Plant Cell Rep. 17: 469–475

    Google Scholar 

  • Rao PS & Bapat VA (1992) Micropropagation of sandalwood (Santalum album L.). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry 18. High-Tech and Micropropagation II (pp 193–210). Springer Verlag, Berlin, Heidelberg

    Google Scholar 

  • Rao PS & Bapat VA (1995) Somatic embryogenesis in sandalwood (Santalum album L.). In: Jain S, Gupta P & Newton R (eds.) Somatic Embryogenesis in Woody Plants, Vol 2 (pp 153–170). Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Rao PS & Rangaswamy NS (1971) Morphogenic studies in tissue culture of parasite Santalum album L. Biologia Plantarum 13: 200–206

    Google Scholar 

  • Srinivasan VV, Ananthapadmanabha HS & Rangaswamy CR (1997) A strategy for sustainable supply of sandal. In: Radomiljac AM, Ananthapadmanabha HS & Rangaswamy CR (eds.) Sandal and Its Products (p. 22). ACIAP Proceedings, Canberra

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Authors and Affiliations

  1. Department of Studies in Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore, India, 570 006

    V. Ravishankar Rai

  2. Biological Sciences, Murdoch University, Perth, WA, 6150, Australia

    Jen McComb

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  1. V. Ravishankar Rai
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  2. Jen McComb
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Correspondence to V. Ravishankar Rai.

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Ravishankar Rai, V., McComb, J. Direct somatic embryogenesis from mature embryos of sandalwood. Plant Cell, Tissue and Organ Culture 69, 65–70 (2002). https://doi.org/10.1023/A:1015037920529

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  • DOI: https://doi.org/10.1023/A:1015037920529

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