Applied Biochemistry and Microbiology

, Volume 37, Issue 3, pp 282-286

First online:

The Respiratory Activity of Rhodococcus rhodochrousM8 Cells Producing Nitrile-Hydrolyzing Enzymes

  • S. M. RogachevaAffiliated withSaratov State University
  • , O. V. IgnatovAffiliated withInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

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The respiratory activity of Rhodococcus rhodochrousM8 cells containing nitrile hydratase and amidase was studied in the presence of nitriles and amides of carbonic acids. The culturing of cells with acrylonitrile and acrylamide yielding maximum respiratory activity was studied. The optimum conditions for measurements and maintenance of respiratory activity were found. Curves for the linear concentration dependence of cell respiratory activity on 0.01–0.5 mM acrylonitrile, 0.025–1.0 mM acetonitrile, and 0.01–0.1 mM acrylamide were plotted. The selectivity of cell respiratory activity for some substrates was analyzed.