Biotechnology Techniques

, Volume 13, Issue 6, pp 391–393

Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

Authors

  • R. Oliveira
    • Centro de Engenharia Biológica — IBQFUniversidade do Minho
  • F. Marques
    • Centro de Engenharia Biológica — IBQFUniversidade do Minho
  • J. Azeredo
    • Centro de Engenharia Biológica — IBQFUniversidade do Minho
Article

DOI: 10.1023/A:1008954301470

Cite this article as:
Oliveira, R., Marques, F. & Azeredo, J. Biotechnology Techniques (1999) 13: 391. doi:10.1023/A:1008954301470

Abstract

The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.

differential precipitationpolysaccharidesproteins

Copyright information

© Kluwer Academic Publishers 1999