Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins
- Cite this article as:
- Oliveira, R., Marques, F. & Azeredo, J. Biotechnology Techniques (1999) 13: 391. doi:10.1023/A:1008954301470
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The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.