Article

Biotechnology Techniques

, Volume 13, Issue 6, pp 391-393

Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

  • R. OliveiraAffiliated withCentro de Engenharia Biológica — IBQF, Universidade do Minho
  • , F. MarquesAffiliated withCentro de Engenharia Biológica — IBQF, Universidade do Minho
  • , J. AzeredoAffiliated withCentro de Engenharia Biológica — IBQF, Universidade do Minho

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Abstract

The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.

differential precipitation polysaccharides proteins