Abstract
The in vitro bulb formation by shoot clumpcultures of N. triandrus on media with jasmonicacid (JA) alone or in association with both2-isopentenyladenine (2-iP) or naphthalene acetic acid(NAA) and on medium with only NAA was studied. Themedia with JA plus 2-iP or JA plus NAA caused a highmultiplication of leaves, significantly higher on thatwith 2-iP. Leaf proliferation was low on medium withJA alone. The media containing JA promoted theformation of a bulb at the base of the leaves and thebulbs attained different sizes. On the medium with JAplus NAA, the number of bulbs that reached up to 5 mmin diameter was higher than that on the medium with JAplus 2-iP, but the highest number was formed on themedium with JA alone. The medium with NAA alone led tothe formation of few small bulbs that were elongatedinstead of roundish as those formed on media with JA.Further culture of the bulbs on a growth mediumsuitable to their enlargement increased their size butthe dimensions attained were dependent upon the mediuminitially used for bulb formation. Bulbs derived frommedium with JA alone attained the largest diameter.Almost all the bulbs grown on the growth medium wererooted. Data described here show that JA promotes in vitro bulb formation in shoot cultures of N.triandrus and suggests that JA might play animportant role in the formation and enlargement ofbulbs in Narcissus plants.
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Abe M, Shibaoka H, Yamane H and Takahashi N (1990) Cell cycle-dependent disruption of microtubules by methyl jasmonate in tobacco BY-2 cells. Protoplasma 156: 1-8
Creelman RA and Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48: 355-381
Chow YN, Selby C and Harvey BMR (1992) Stimulation by sucrose of Narcissus bulbil formation in vitro. J Hort Sci 67(2): 289-293
Hussey G (1982) In vitro propagation of Narcissus. 1982. Ann Bot 49: 707-719
Koda Y and Kikuta Y (1991) Possible involvement of jasmonic acid in tuberization in yam plants. Plant Cell Physiol 32: 629-633
Koda Y (1997) Possible involvement of jasmonates in various morphogenic events. Physiol Plant 100: 639-946
Matsuki T, Tazaki H, Fujimori T and Hogetsu T (1992) The influences of jasmonic acid methyl ester on microtubules in potato cells and formation of potato tubers. Biosci Biotech Biochem 56: 1329-1330
Mita T and Shibaoka H (1983) Changes in microtubules in onion leaf sheath cells during bulb development. Plant Cell Physiol 24: 109-117
Miyamoto K, Oka M and Ueda J (1997) Update on the possible mode of action of the jasmonates: Focus on the metabolism of cell wall polysaccharides in relation to growth and development. Physiol Plant 100: 631-638
Nojiri H, Yamane H, Seto H, Yamaguchi I, Murofushi N, Yoshihara T and Shibaoka H (1992) Qualitative and quantitative analysis of endogenous jasmonic acid in bulbing and non-bulbing onion plants. Plant Cell Physiol 33: 1225-1231
Ravnikar M, Vilhar B and Gogala N (1992) Stimulatory effect of jasmonic acid on potato stem node and protoplast culture. J Plant Growth Regul 11: 29-33
Ravnikar M, Zel J, Plaper I and Spacapan A (1993) Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J Plant Growth Regul 12: 73-77
Santos J, Santos I and Salema R (1998) In vitro production of bulbs of Narcissus bulbocodium flowering in the first season of growth. Scientia Hort 76: 205-217
Seabrook JEA (1990) Narcissus (Daffodil). In: Ammirato PV, Evans DA, Sharp WR and Bajaj PS (eds) Handbook of Plant Cell Culture, vol. 5, pp 577-597. New York: McGraw-Hill
Squires WM and Langton FA (1990) Potential and limitations of Narcissus micropropagation: an experimental evaluation. Acta Horticulturae 266: 67-75
Squires WM, Langton FA and Fenlon JS (1991) Factors influencing the transplantation success of micropropagated narcissus bulbils. J Horticult Sci 66(6): 661-671
Steinitz B and Yahel H. (1982) In vitro propagation of Narcissus tazetta. HortScience 17(3): 333-334
Van den Berg JH and Ewing EE (1991) Jasmonates and their role in plant growth and development, with special references to the control of potato tuberization: a review. Amer Potato J 68: 781-794
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Santos, I., Salema, R. Promotion by Jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L.. Plant Growth Regulation 30, 133–138 (2000). https://doi.org/10.1023/A:1006381127884
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DOI: https://doi.org/10.1023/A:1006381127884