World Journal of Microbiology and Biotechnology

, 20:535

RFLP of 16S-ITSrDNA Region to Differentiate Lactobacilli at Species Level

  • Elif Yavuz
  • Hatice Gunes
  • Cisem Bulut
  • Sebnem Harsa
  • A. Fazil Yenidunya
Article

DOI: 10.1023/B:WIBI.0000043151.09366.d7

Cite this article as:
Yavuz, E., Gunes, H., Bulut, C. et al. World Journal of Microbiology and Biotechnology (2004) 20: 535. doi:10.1023/B:WIBI.0000043151.09366.d7

Abstract

The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and TaqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.

Lactic acid bacteriarestriction fragment length polymorphismsimilarity analysis

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Elif Yavuz
    • 1
  • Hatice Gunes
    • 2
  • Cisem Bulut
    • 1
  • Sebnem Harsa
    • 1
  • A. Fazil Yenidunya
    • 2
  1. 1.Biotechnology and Bioengineering ProgrammeIzmir Institute of TechnologyGulbahceTurkey
  2. 2.Department of BiologyScience Faculty, Izmir Institute of TechnologyGulbahceTurkey