RFLP of 16S-ITSrDNA Region to Differentiate Lactobacilli at Species Level
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- Yavuz, E., Gunes, H., Bulut, C. et al. World Journal of Microbiology and Biotechnology (2004) 20: 535. doi:10.1023/B:WIBI.0000043151.09366.d7
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The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and TaqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.