Article

World Journal of Microbiology and Biotechnology

, Volume 20, Issue 6, pp 535-537

RFLP of 16S-ITSrDNA Region to Differentiate Lactobacilli at Species Level

  • Elif YavuzAffiliated withBiotechnology and Bioengineering Programme, Izmir Institute of Technology
  • , Hatice GunesAffiliated withDepartment of Biology, Science Faculty, Izmir Institute of Technology
  • , Cisem BulutAffiliated withBiotechnology and Bioengineering Programme, Izmir Institute of Technology
  • , Sebnem HarsaAffiliated withBiotechnology and Bioengineering Programme, Izmir Institute of Technology
  • , A. Fazil YenidunyaAffiliated withDepartment of Biology, Science Faculty, Izmir Institute of Technology

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Abstract

The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and TaqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.

Lactic acid bacteria restriction fragment length polymorphism similarity analysis