Veterinary Research Communications

, Volume 29, Issue 3, pp 263–269

Detection of Porcine Circovirus Type 2, Porcine Parvovirus and Porcine Pseudorabies Virus from Pigs with Postweaning Multisystemic Wasting Syndrome by Multiplex PCR

  • S. Cao
  • H. Chen
  • J. Zhao
  • J. Lü
  • S. Xiao
  • M. Jin
  • A. Guo
  • B. Wu
  • Q. He
Article

DOI: 10.1023/B:VERC.0000047501.78615.0b

Cite this article as:
Cao, S., Chen, H., Zhao, J. et al. Vet Res Commun (2005) 29: 263. doi:10.1023/B:VERC.0000047501.78615.0b

Abstract

Multiplex PCR was established to detect porcine circovirus type 2 (PCV-2), porcine parvovirus (PPV) and porcine pseudorabies virus (PRV) and applied to samples from 137 piglets exhibiting clinical signs of postweaning multisystemic wasting syndrome (PMWS). PCV-2 DNA was detected from all samples. Moreover, 43 samples were positive for PPV but negative for PRV; 11 samples were positive for PRV but negative for PPV; and 35 samples were positive both for PPV and PRV. These results suggests that PCV-2 co-infection with PRV and PPV may play an important role in PMWS. Also, multiplex PCR is an appropriate candidate method for diagnosis of PCV-2, PRV and PPV simultaneously in field cases.

multiplex PCRporcine circovirus type 2porcine parvovirusporcine pseudorabies viruspostweaning multisystemic wasting syndrome

Copyright information

© Springer 2005

Authors and Affiliations

  • S. Cao
    • 1
  • H. Chen
    • 1
  • J. Zhao
    • 1
  • J. Lü
    • 1
  • S. Xiao
    • 1
  • M. Jin
    • 1
  • A. Guo
    • 1
  • B. Wu
    • 1
  • Q. He
    • 1
  1. 1.Laboratory of Animal Virology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhan, HubeiP.R. China