Veterinary Research Communications

, Volume 28, Issue 6, pp 543–552

Partial Antigenic Characterization of Buffalopox Virus

  • P. Anand Kumar
  • G. Butchaiah
Article

DOI: 10.1023/B:VERC.0000040245.85281.9a

Cite this article as:
Anand Kumar, P. & Butchaiah, G. Vet Res Commun (2004) 28: 543. doi:10.1023/B:VERC.0000040245.85281.9a

Abstract

The present study was undertaken to antigenically characterize the buffalopox virus (BPV). Six monoclonal antibodies (MAbs) against the BP4 strain of BPV have been produced and characterized. All six MAbs appeared to be specific to BPV, as none of them showed cross-reactivity with other poxviruses in antigen capture ELISA. Only two MAbs (20AB8 and 20CD11) bound significantly with different BPV isolates in antigen capture ELISA, whereas the remaining four MAbs bound weakly with the BPV. In Western blot analysis with purified BPV-BP4, the rabbit hyperimmune serum against purified BPV-BP4 reacted with 15 immunodominant polypeptides (100 kDa to 25 kDa), whereas two MAbs (21CB6, 21DB11) reacted with 42 kDa and 45 kDa polypeptides, respectively. However, three MAbs (20AB8, 20CD11, 21CB5) reacted with three degraded polypeptides (100 kDa, 40 kDa and 87 kDa) of BPV-BP4. In radioimmunoprecipitation assay (RIPA) with the rabbit hyperimmune serum to BPV-BP4, three virus specific polypeptides (69 kDa, 34 kDa, 32 kDa) were recognized in BPV-BP4, whereas two polypeptides (69 kDa, 34 kDa) were recognized in other BPV isolates (BPV-Bly, BPV-Vij96, BPV-Vij97). In virus neutralization test, none of the six MAbs tested showed any significant neutralizing ability to infection with different BPV isolates. However, the hyperimmune serum showed weak neutralizing ability to BPV infection.

antigenic characterizationbuffalopox virusELISAmonoclonal antibodiesradioimmunoprecipitation assayWestern blot

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • P. Anand Kumar
    • 1
  • G. Butchaiah
    • 1
  1. 1.National Biotechnology CentreIndian Veterinary Research Institute, IzatnagarUPIndia