Abstract
An investigation was carried out to study the antibiotic sensitivity of avian strains ofPasteurella multocida and to select an effective antimicrobial agent for control of avian pasteurellosis in India. A total of 123 strains ofP. multocida recently isolated from different avian species (chicken, duck, turkey, quail, and goose), from different regions of India were subjected to antibiotic sensitivity tests using 20 different antibiotics. Absolute resistance was observed against sulfadiazine. The studies indicated that the strains were most sensitive to chloramphenicol (73.98%), followed by enrofloxacin (71.54%), lincomycin (64.23%), norfloxacin (61.79%) and doxycycline-HCl (56.91%). The majority of the strains were found to exhibit intermediate sensitivity. Chloramphenicol was selected and suggested for treatment. Antibiogram studies also revealed the emergence of multidrug-resistant strains ofP. multocida among Indian poultry.
Similar content being viewed by others
REFERENCES
Berman, S.M. and Hirsh, D.C., 1978. Partial characterization of R plasmids from Pasteurella multocida strains from turkeys. Antimicrobial Agents and Chemotherapy, 14, 348–352
Carter, G.R. and Subronto, P., 1973. Identification of type D strains of Pasteurella multocida with acriflavin. American Journal of Veterinary Research, 34, 293–294
Christensen, J.P. and Bisgaard, M., 2000. Fowl cholera. Reviews in Scientifique et Technique de l'Offce International des Epizooties, 19, 626–637
Cote, S., Harrel, J., Higgens, R. and Jacques, H., 1991. Resistance to antimicrobial agents and prevalence of R plasmids in Pasteurella multocida from swine. American Journal of Veterinary Research, 52,1653–1657
Cowan, S.T. and Steel, K.J., 1970. Manual for the Identification of Medical Bacteria, (Cambridge University Press, Cambridge)
Cruickshank, R., Duguid, J.P., Marmion, B.P. and Swain, R.H.A., 1975. Medical Microbiology, 2nd edn, vol 1, (Churchill Livingstone, Edinburgh)
Donahue, J.M. and Olson, L.D., 1972. The in vitro sensitivity of Pasteurella multocida of turkey origin to various chemotherapeutic agents. Avian Diseases, 12, 506–511
Fales, W.H., Selby, L.A., Weber, J.J., Hoffman, L.J., Hintner, S.L., Nelson, J.L., Miller, R.B., Thorne, J.G., McGinity, J.T. and Smith, D.K., 1982. Antimicrobial resistance among Pasteurella sp. recovered from Missouri and Iowa cattle with bovine respiratory disease complex. Journal of the American Veterinary Medical Association, 181, 477–479
Hirsh, D.C., Martin, L.D. and Rhodes, K.R., 1981. Conjugal transfer of R-plasmid in Pasteurella multocida. Antimicrobial Agents and Chemotherapy, 20, 415–417
Hirsh, D.C., Martin, L.D. and Rhoades, K.R., 1985. Resistance plasmids of Pasteurella multocida strains from turkeys. American Journal of Veterinary Research, 46, 1490–1493
Hirsh, D.C., Hansen, L.M., Dorfman, L.C., Snipes, R.P., Carpenter, T.E., Hird, D.W. and McCapes, R.P., 1989. Resistance to antimicrobial agents and prevalence of R-plasmids in P.multocida from turkeys. Antimicrobial Agents and Chemotherapy, 33, 670–673
Hormansdor, F.S. and Bauer, J., 1996. Resistance pattern of bovine Pasteurella. Berliner und Muünchener Tierarztliche Wochenschrift, 109, 168–171
Ishii, H., Mokudai, K., Tetsuya, S., Masumoto, T., Kameda, M., Kurihara, O., Arak, Y., Ijobe, S. and Hashimoto, H., 1990. Drug susceptibility in Pasteurella multocida strains from swine from 1987 to 1989. Japanese Journal of Veterinary Science, 52, 399–402
Jonas, M., Morishita, T.Y., Angrick, E.J. and Fahya, J., 2001. Characterization of nine Pasteurella multocida strains from avian cholera outbreaks in Indonesia. Avian Diseases, 45, 34–42
Kulkarni, D.D., Karpe, A.G., Bannalikar, A.S. and Gujar, M.B., 1990. Biological observation on pasteurellosis in domestic animals and poultry. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 11,22
Mehrotra, P.K. and Bhargava, S., 1999. A note on characterisation and antibiotic sensitivity of Pasteurella strains from domestic animals. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 20, 144–146
Madhekar, D., Satyanarayana, N.V.V. and Rao, C.R.S., 1982. Incidence of poultry diseases in Krishna district of Andhra Pradesh. Poultry Advisor, XVI,61–64
Rajini, R., Seshagiri Rao, A., Dhanalkshmi, K. and Sarma, B.J.R., 1995. Studies on avian pasteurellosis in Andhra Pradhesh. Indian Veterinary Journal, 72, 115–118
Ramasastry, P. and Ramarao, M., 1989. Acute pasteurellosis in ducks report on an outbreak and its control. Poultry Advisor, XXII, 43–44
Rimler, R.B. and Glisson, J.R., 1997. Fowl cholera. In: B.W. Calnek, H.J. Barnes, C.W. Beard, L.R. Mcdougald and Y.M. Saif (eds), Diseases of Poultry, 10th edn., (Iowa State University Press, Ames, IA), 143–159
Sharma, K.N., Mehrotra, P.K. and Khanna, V.K., 1979. A note on characterization and antibiotics sensitivity of pasteurellae. Indian Journal of Animal Science, 49,142
Silver, R.P., Leming, B., Garon, C.F. and Hgerpe, C.H., 1979. R-plasmids in Pasteurella multocida. Plasmid, 2, 493–497
Stone, A.B., 1975. R-factors plasmids conferring resistance of antibacterial agents. Science Progress, 62,89
Townsend, K.M., Frost, A.J. Lee, C.W., Papadimitrious, J.M. and Dawkins, H.J., 1998. Development of PCR assays for species and type specific identification of Pasteurella multocida strains. Journal of Clinical Microbiology, 36, 1096–1100
Walser, M.M and Davis, B., 1975. In vitro characterization of field isolates of Pasteurella multocida from Georgia turkeys. Avian Diseases, 19, 525–531
Wilson, K., 1987. Preparation of genomic DNA from bacteria. In: F.M. Ausubal, R. Brent, R.L. Kirston, D.D., Moore, J.G. Seidman, J.A., Smith and K. Struhl (eds), Current Protocols in Molecular Biology, vol. 1, (John Wiley, New York), 2.4.1–2.4.2.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shivachandra, S., Kumar, A., Biswas, A. et al. Antibiotic Sensitivity Patterns among Indian Strains of Avian Pasteurella multocida . Tropical Animal Health and Production 36, 743–750 (2004). https://doi.org/10.1023/B:TROP.0000045950.35070.7f
Issue Date:
DOI: https://doi.org/10.1023/B:TROP.0000045950.35070.7f