Article

Transgenic Research

, Volume 12, Issue 6, pp 743-746

First online:

Utility of a C57BL/6 ES line versus 129 ES lines for Targeted Mutations in Mice

  • Carol B. WareAffiliated withDepartment of Comparative Medicine, University of Washington Email author 
  • , La' Akea N. SivertsAffiliated withDepartment of Comparative Medicine, University of Washington
  • , Angelique M. NelsonAffiliated withDepartment of Comparative Medicine, University of Washington
  • , John F. MortonAffiliated withDepartment of Comparative Medicine, University of Washington
  • , Warren C. LadigesAffiliated withDepartment of Comparative Medicine, University of Washington

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Abstract

Inbred ES lines, though useful for generating targeted mutations in mice, are used infrequently. To appreciate the relative efficiency of inbred ES lines, a C57BL/6 ES line was compared with 129 strain ES lines for effectiveness in chimera formation leading to the establishment of targeted mutations in mice. Data from a transgenic facility spanning 7 years were collected. C57BL/6 ES cells injected into Balb/c embryos results in lower coat color chimerism than do 129 ES cells injected into C57BL/6 embryos. Combined data indicate that five independent targeted C57BL/6 clones should be injected as compared to three independent 129 clones to generate enough chimeras to effectively test for germ-line transmission. Thus, although less efficient than 129 ES lines, the C57BL/6 ES line is a relatively competent line and useful for the routine generation of targeted mutations in mice on a defined genetic background.

C57BL/6 ES cells mice targeted mutation