Transgenic Research

, Volume 12, Issue 6, pp 715–722

Transgenic Tobacco Expressing Pinellia ternata Agglutinin Confers Enhanced Resistance to Aphids

  • Jianhong Yao
  • Yongzhen Pang
  • Huaxiong Qi
  • Bingliang Wan
  • Xiuyun Zhao
  • Weiwen Kong
  • Xiaofen Sun
  • Kexuan Tang
Article

DOI: 10.1023/B:TRAG.0000005146.05655.7d

Cite this article as:
Yao, J., Pang, Y., Qi, H. et al. Transgenic Res (2003) 12: 715. doi:10.1023/B:TRAG.0000005146.05655.7d

Abstract

Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) viaAgrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that the pta gene had integrated into the plant genome and northern blot analysis revealed transgene expression at various levels in transgenic plants. Genetic analysis confirmed Mendelian segregation of the transgene in T1 progeny. Insect bioassays showed that transgenic plants expressing PTA inhibited significantly the growth of peach potato aphid

(Myzus persicae Sulzer). This is the first report that transgenic plants expressing pta confer enhanced resistance to aphids. Our study indicates that the pta gene can be used as a supplement to the snowdrop (Galanthus nivalis) lectin gene (gna) in the control of aphids, a sap-sucking insect pest causing significant yield losses of crops.

insect bioassay peach potato aphid (Myzus persicae Sulzer) Pinellia ternata agglutinin (PTA) transformation transgenic tobacco 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Jianhong Yao
    • 1
  • Yongzhen Pang
    • 1
  • Huaxiong Qi
    • 2
  • Bingliang Wan
    • 2
  • Xiuyun Zhao
    • 1
  • Weiwen Kong
    • 1
  • Xiaofen Sun
    • 1
  • Kexuan Tang
    • 1
  1. 1.State Key Laboratory of Genetic EngineeringSchool of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan UniversityShanghaiPR China
  2. 2.Hubei Academy of Agricultural SciencesWuhanPR China