, Volume 52, Issue 9, pp 2073-2077

Immobilization of trypsin on polysaccharides upon intense mechanical treatment

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Abstract

Immobilization of a ptoteolytic enzyme, trypsin, on chitosan and cellulose was accomplished in the solid state under pressure and shear strain. Trypsin immobilized in this way retains 70—80% of the catalytic activity and exhibits prolonged action. The mechanical treatment allows introduction of a much larger amount of the active protein into a polymer matrix than the equilibrium sorption from solution.