, Volume 82, Issue 1, pp 73-81

A Simple Alternative Approach to Assessing the Fate of Absorbed Light Energy Using Chlorophyll Fluorescence

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We propose a simplified alternative method for quantifying the partitioning of excitation energy between photochemistry, fluorescence and thermal dissipation. This alternative technique uses existing well-defined quantum efficiencies such as ΦPS II, leaving no 'excess' efficiency unaccounted for, effectively separates regulated and constitutive thermal dissipation processes, does not require the use of Fo and F′o measurements and gives very similar results to the method proposed by Kramer et al. [(2004) Photosynth Res 79: 209–218]. We demonstrate the use of the technique using chlorophyll fluorescence measurements in grapevine leaves and observe a high dependence on thermal dissipation processes (up to 75%) at both high light and low temperature.

This revised version was published online in June 2006 with corrections to the Cover Date.