Article

Photosynthesis Research

, Volume 81, Issue 1, pp 41-47

Superoxide, Hydrogen Peroxide and Hydroxyl Radical in D1/D2/cytochrome b-559 Photosystem II Reaction Center Complex

  • Ke LiuAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Jian SunAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Yu-guang SongAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Bin LiuAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Ying-kai XuAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Shu-xiao ZhangAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Qiu TianAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences
  • , Yang LiuAffiliated withState Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences

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Abstract

Spin-trapping electron spin resonance (ESR) was used to monitor the formation of superoxide and hydroxyl radicals in D1/D2/cytochrome b-559 Photosystem II reaction center (PS II RC) Complex. When the PS II RC complex was strongly illuminated, superoxide was detected in the presence of ubiquinone. SOD activity was detected in the PS II RC complex. A primary product of superoxide, hydrogen peroxide, resulted in the production of the most destructive reactive oxygen species, •OH, in illuminated PS II RC complex. The contributions of ubiquinone, SOD and H2O2 to the photobleaching of pigments and protein photodamage in the PS II RC complex were further studied. Ubiquinone protected the PS II RC complex from photodamage and, interestingly, extrinsic SOD promoted this damage. All these results suggest that PS II RC is an active site for the generation of superoxide and its derivatives, and this process protects organisms during strong illumination, probably by inhibiting more harmful ROS, such as singlet oxygen.

hydroxyl radical Photosystem II reaction center superoxide superoxide dismutase