Plant Molecular Biology

, Volume 54, Issue 1, pp 1–9

A novel zinc-finger protein with a proline-rich domain mediates ABA-regulated seed dormancy in Arabidopsis

  • Yuehui He
  • Susheng Gan
Article

DOI: 10.1023/B:PLAN.0000028730.10834.e3

Cite this article as:
He, Y. & Gan, S. Plant Mol Biol (2004) 54: 1. doi:10.1023/B:PLAN.0000028730.10834.e3

Abstract

Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATOR OF ABA-REGULATED DORMANCY 1(MARD1) was identified and functionally analyzed. MARD1expression is up-regulated by ABA. A T-DNA insertion in the promoter region downstream of two ABA-responsive elements (ABREs) renders MARD1unable to respond to ABA. The mard1 seeds are less dormant and germinate in total darkness; their germination is resistant to external ABA at the stage of radicle protrusion. These results suggest that this novel zinc-finger protein with a proline-rich N-terminus is an important downstream component of the ABA signaling pathway that mediates ABA-regulated seed dormancy in Arabidopsis.

abscisic acid MARD1 seed dormancy seed germination 

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Yuehui He
    • 1
  • Susheng Gan
    • 1
    • 2
  1. 1.Plant Physiology/Biochemistry/Molecular Biology ProgramUniversity of KentuckyLexingtonUSA
  2. 2.Cornell Genomics Initiative and Department of HorticultureIthacaUSA
  3. 3.Department of BiochemistryUniversity of Wisconsin-MadisonMadisonUSA

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