Pharmaceutical Research

, Volume 21, Issue 4, pp 641–648

The Characteristics and Mechanisms of Uptake of PLGA Nanoparticles in Rabbit Conjunctival Epithelial Cell Layers

  • Mohamed G. Qaddoumi
  • Hideo Ueda
  • Johnny Yang
  • Jasmine Davda
  • Vinod Labhasetwar
  • Vincent H. L. Lee
Article

DOI: 10.1023/B:PHAM.0000022411.47059.76

Cite this article as:
Qaddoumi, M.G., Ueda, H., Yang, J. et al. Pharm Res (2004) 21: 641. doi:10.1023/B:PHAM.0000022411.47059.76

Abstract

Purpose. To delineate the characteristics and mechanisms of up- take of biodegradable poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles in primary cultured rabbit conjunctival epithelial cells (RCECs).

Methods. Poly(d,l-lactide-co-glycolide) nanoparticles (PLGA 50:50, 100 nm in diameter) containing 6-coumarin (as a fluorescent marker) were used. The effect of size was studied using various particle sizes (100 nm, 800 nm, and 10 μm). The effect of cytochalasin D, nocodazole, and metabolic inhibitors on nanoparticle uptake was investigated. The capability of nanoparticles to enhance the uptake of an encapsulated protein, BSA bound to Texas red (TR-BSA), was evaluated.

Results. Maximal uptake of nanoparticles at 37°C occurred at 2 h, and 100-nm particles had the highest uptake in RCECs in comparison with 800-nm and 10-μm particles. Nanoparticle uptake was saturable over the 0.1-4 mg/ml concentration range. Nanoparticle uptake was confirmed by confocal microscopy and was inhibited significantly by coumarin-free nanoparticles (of similar size), by lower incubation temperature, and by the presence of metabolic inhibitors and cytochalasin D. The uptake of encapsulated TR-BSA in RCECs at 4 h was 28% higher than free BSA application.

Conclusion. Our findings suggest that PLGA nanoparticle uptake in primary cultured rabbit conjunctival epithelial cells occurs most likely by adsorptive-type endocytosis.

absorption endocytosis ocular drug delivery ophthalmic polymeric microparticles. 

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Mohamed G. Qaddoumi
    • 1
  • Hideo Ueda
    • 1
  • Johnny Yang
    • 1
  • Jasmine Davda
    • 3
  • Vinod Labhasetwar
    • 3
    • 4
  • Vincent H. L. Lee
    • 1
    • 2
  1. 1.Department of Pharmaceutical SciencesUniversity of Southern CaliforniaLos Angeles
  2. 2.Department of OphthalmologyUniversity of Southern CaliforniaLos Angeles
  3. 3.Department of Pharmaceutical SciencesUniversity of Nebraska Medical CenterOmaha
  4. 4.Biochemistry and Molecular BiologyUniversity of Nebraska Medical CenterOmaha