Neurochemical Research

, Volume 29, Issue 6, pp 1161–1168

Expression Profile Analysis of Neurodegenerative Disease: Advances in Specificity and Resolution

  • Jason G. Glanzer
  • Phillip G. Haydon
  • James H. Eberwine
Article

DOI: 10.1023/B:NERE.0000023603.17615.8c

Cite this article as:
Glanzer, J.G., Haydon, P.G. & Eberwine, J.H. Neurochem Res (2004) 29: 1161. doi:10.1023/B:NERE.0000023603.17615.8c

Abstract

Microarray technology has become a common tool for developing expression profiles. Initially used in the analysis of cells lines and homogeneous tissues, this platform has been applied to more diverse tissues, such as the brain. Several neural disorders have already been profiled by microarrays using relatively large amounts of tissue. This data has unveiled many genes with differential expression between normal and diseased tissue that could potentially be used as gene markers for these afflictions. Because of the heterogeneity of the CNS, it is likely that small differences between gene expression in these studies would be enhanced by the sampling of a subset of cells based on these newly characterized gene markers. Subtraction of normal, unaffected cells from the sample may also result in a more accurate profile of a diseased cell. Expression profile studies from several neuropathological states are presented, with emphasis placed on those studies using small samples of cellular material and those using specialized methods of cell isolation and RNA amplification

AIDS Alzheimer aRNA dementia expression profile microarray Parkinson schizophrenia single cell 

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Jason G. Glanzer
    • 1
  • Phillip G. Haydon
    • 2
  • James H. Eberwine
    • 1
  1. 1.Department of PharmacologyUniversity of Pennsylvania Medical CenterPhiladelphia
  2. 2.Department of NeuroscienceUniversity of Pennsylvania Medical CenterPhiladelphia

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