Neurochemical Research

, Volume 29, Issue 6, pp 1129–1144

Comprehensive Proteome Expression Profiling of Undifferentiated versus Differentiated Neural Stem Cells from Adult Rat Hippocampus

  • Martin H. Maurer
  • Robert E. FeldmannJr.
  • Carsten D. Fütterer
  • Jo Butlin
  • Wolfgang Kuschinsky
Article

DOI: 10.1023/B:NERE.0000023600.25994.11

Cite this article as:
Maurer, M.H., Feldmann, R.E., Fütterer, C.D. et al. Neurochem Res (2004) 29: 1129. doi:10.1023/B:NERE.0000023600.25994.11

Abstract

Adult neural stem cells can be isolated from various regions of the rat brain and seem to have multilineage differentiation potential. In this study, we investigated the hypothesis that global protein expression of adult neural stem cells isolated from rat hippocampus is changed during in vitro differentiation. After 2 days of differentiation, we separated total protein extracts by two-dimensional gel electrophoresis and used mass spectrometry and gel-matching for identification. We detected a differential expression in 367 regulated protein spots, of which 128 could be identified. Regulated proteins included participants in transcription and DNA metabolism; signal transduction and Ca2+-signaling; MAP kinase pathways; cytoskeletal rearrangement; regulation of cell cycle, proliferation, and survival; protein biosynthesis, folding, and degradation; and glycine and glutamate metabolic pathways. These results suggest a massive reorganization of the stem cell proteomic profile upon differentiation and indicate potential cellular targets mediating the differentiation of neural stem cells.

Adult neural stem cells differentiation two-dimensional gel electrophoresis hippocampus mass spectrometry proteomics rat 

Copyright information

© Plenum Publishing Corporation 2004

Authors and Affiliations

  • Martin H. Maurer
    • 1
  • Robert E. FeldmannJr.
    • 1
  • Carsten D. Fütterer
    • 2
  • Jo Butlin
    • 3
  • Wolfgang Kuschinsky
    • 1
  1. 1.Department of Physiology and PathophysiologyUniversity of HeidelbergHeidelbergGermany
  2. 2.Department of Anesthesiology and Surgical Intensive Care, Faculty of Clinical MedicineUniversity of HeidelbergMannheimGermany
  3. 3.PerkinElmer Life & Analytical SciencesCambridgeUnited Kingdom

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